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Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis.

Publication ,  Journal Article
Sinclair, EM; Cohen, CC; Tran, V; Jones, DP; Alvarez, JA; Kamaleswaran, R; Rad, MG; Kruszewski, PG; Vos, MB
Published in: J Pediatr Gastroenterol Nutr
March 1, 2023

BACKGROUND/OBJECTIVES: Eosinophilic esophagitis (EoE) is an inflammatory disease of unclear etiology. The aim of this study was to use untargeted plasma metabolomics to identify metabolic pathway alterations associated with EoE to better understand the pathophysiology. METHODS: This prospective, case-control study included 72 children, aged 1-17 years, undergoing clinically indicated upper endoscopy (14 diagnosed with EoE and 58 controls). Fasting plasma samples were analyzed for metabolomics by high-resolution dual-chromatography mass spectrometry. Analysis was performed on sex-matched groups at a 2:1 ratio. Significant differences among the plasma metabolite features between children with and without EoE were determined using multivariate regression analysis and were annotated with a network-based algorithm. Subsequent pathway enrichment analysis was performed. RESULTS: Patients with EoE had a higher proportion of atopic disease (85.7% vs 50%, P = 0.019) and any allergies (100% vs 57.1%, P = 0.0005). Analysis of the dual chromatography features resulted in a total of 918 metabolites that differentiated EoE and controls. Glycerophospholipid metabolism was significantly enriched with the greatest number of differentiating metabolites and overall pathway enrichment ( P < 0.01). Multiple amino and fatty acid pathways including linoleic acid were also enriched, as well as pyridoxine metabolism ( P < 0.01). CONCLUSIONS: In this pilot study, we found differences in metabolites involved in glycerophospholipid and inflammation pathways in pediatric patients with EoE using untargeted metabolomics, as well as overlap with amino acid metabolome alterations found in atopic disease.

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Published In

J Pediatr Gastroenterol Nutr

DOI

EISSN

1536-4801

Publication Date

March 1, 2023

Volume

76

Issue

3

Start / End Page

355 / 363

Location

United States

Related Subject Headings

  • Prospective Studies
  • Pilot Projects
  • Metabolomics
  • Humans
  • Gastroenterology & Hepatology
  • Gastritis
  • Eosinophilic Esophagitis
  • Eosinophilia
  • Enteritis
  • Child
 

Citation

APA
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Sinclair, E. M., Cohen, C. C., Tran, V., Jones, D. P., Alvarez, J. A., Kamaleswaran, R., … Vos, M. B. (2023). Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis. J Pediatr Gastroenterol Nutr, 76(3), 355–363. https://doi.org/10.1097/MPG.0000000000003693
Sinclair, Elizabeth M., Catherine C. Cohen, ViLinh Tran, Dean P. Jones, Jessica A. Alvarez, Rishikesan Kamaleswaran, Milad Ghiasi Rad, Patrice G. Kruszewski, and Miriam B. Vos. “Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis.J Pediatr Gastroenterol Nutr 76, no. 3 (March 1, 2023): 355–63. https://doi.org/10.1097/MPG.0000000000003693.
Sinclair EM, Cohen CC, Tran V, Jones DP, Alvarez JA, Kamaleswaran R, et al. Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis. J Pediatr Gastroenterol Nutr. 2023 Mar 1;76(3):355–63.
Sinclair, Elizabeth M., et al. “Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis.J Pediatr Gastroenterol Nutr, vol. 76, no. 3, Mar. 2023, pp. 355–63. Pubmed, doi:10.1097/MPG.0000000000003693.
Sinclair EM, Cohen CC, Tran V, Jones DP, Alvarez JA, Kamaleswaran R, Rad MG, Kruszewski PG, Vos MB. Untargeted, High-Resolution Metabolomics in Pediatric Eosinophilic Esophagitis. J Pediatr Gastroenterol Nutr. 2023 Mar 1;76(3):355–363.

Published In

J Pediatr Gastroenterol Nutr

DOI

EISSN

1536-4801

Publication Date

March 1, 2023

Volume

76

Issue

3

Start / End Page

355 / 363

Location

United States

Related Subject Headings

  • Prospective Studies
  • Pilot Projects
  • Metabolomics
  • Humans
  • Gastroenterology & Hepatology
  • Gastritis
  • Eosinophilic Esophagitis
  • Eosinophilia
  • Enteritis
  • Child