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Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2.

Publication ,  Journal Article
Wang, C; Chang, C-C; Chi, J-T; Yuan, F
Published in: Pharmaceutics
March 29, 2024

Our previous study discovered that sucrose and other non-reducing sugars (e.g., trehalose and raffinose) could be used to improve the electrotransfer (ET) of molecular cargo, including DNA, mRNA, and ribonucleoprotein in various cell lines and primary human cells in vitro and in vivo. To understand the molecular mechanisms of this improvement, we used RNA sequencing technology to analyze changes in the cell transcriptome after sucrose treatment. The results from our analysis demonstrated that the sucrose treatment upregulated phospholipase A2 and V-ATPase gene families, which could potentially influence the acidity of intracellular vesicles through augmenting vesicle fusion and the influx of proton, respectively. To determine how this upregulation affects ET efficiency, we treated cells with pharmaceutical inhibitors of phospholipase A2 and V-ATPase. The data demonstrated that the treatment with the phospholipase A2 inhibitor could reverse the ET improvement elicited by the sucrose treatment. The V-ATPase inhibitor treatment either had little influence or further enhanced the effect of the sucrose treatment on the ET efficiency. These observations provide a molecular explanation for our previous findings, demonstrating that the sucrose treatment primarily enhanced the ET efficiency by promoting vesicle trafficking and fusion through the activation of phospholipase A2.

Duke Scholars

Published In

Pharmaceutics

DOI

ISSN

1999-4923

Publication Date

March 29, 2024

Volume

16

Issue

4

Location

Switzerland

Related Subject Headings

  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences
 

Citation

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Wang, C., Chang, C.-C., Chi, J.-T., & Yuan, F. (2024). Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2. Pharmaceutics, 16(4). https://doi.org/10.3390/pharmaceutics16040475
Wang, Chunxi, Chun-Chi Chang, Jen-Tsan Chi, and Fan Yuan. “Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2.Pharmaceutics 16, no. 4 (March 29, 2024). https://doi.org/10.3390/pharmaceutics16040475.
Wang C, Chang C-C, Chi J-T, Yuan F. Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2. Pharmaceutics. 2024 Mar 29;16(4).
Wang, Chunxi, et al. “Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2.Pharmaceutics, vol. 16, no. 4, Mar. 2024. Pubmed, doi:10.3390/pharmaceutics16040475.
Wang C, Chang C-C, Chi J-T, Yuan F. Sucrose Treatment Enhances the Electrotransfer of DNA by Activating Phospholipase A2. Pharmaceutics. 2024 Mar 29;16(4).

Published In

Pharmaceutics

DOI

ISSN

1999-4923

Publication Date

March 29, 2024

Volume

16

Issue

4

Location

Switzerland

Related Subject Headings

  • 3214 Pharmacology and pharmaceutical sciences
  • 1115 Pharmacology and Pharmaceutical Sciences