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Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization.

Publication ,  Journal Article
Curvino, EJ; Woodruff, ME; Roe, EF; Freire Haddad, H; Cordero Alvarado, P; Collier, JH
Published in: ACS biomaterials science & engineering
May 2024

Oral immunization is a promising strategy for preventing and treating gastrointestinal (GI) infections and diseases, as it allows for direct access to the disease site. To elicit immune responses within the GI tract, however, there are many obstacles that oral vaccines must surmount, including proteolytic degradation and thick mucus barriers. Here, we employed a modular self-assembling peptide nanofiber platform to facilitate oral immunization against both peptide and small molecule epitopes. Synthesizing nanofibers with d-amino acids rendered them resistant to proteases in vitro, whereas l-amino acid nanofibers were rapidly degraded. Additionally, the inclusion of peptide sequences rich in proline, alanine, and serine (PAS), increased nanofiber muco-penetration, and accelerated nanofiber transport through the GI tract. Oral immunization with PASylated nanofibers and mucosal adjuvant generated local and systemic immune responses to a peptide epitope but only for l-amino acid nanofibers. Further, we were able to apply this design to also enable oral immunization against a small molecule epitope and illustrated the therapeutic and prophylactic effectiveness of these immunizations in mouse models of colitis. These findings demonstrate that supramolecular peptide self-assemblies have promise as oral vaccines and immunotherapies.

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Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

May 2024

Volume

10

Issue

5

Start / End Page

3041 / 3056

Related Subject Headings

  • Peptides
  • Nanofibers
  • Mice, Inbred C57BL
  • Mice
  • Immunization
  • Female
  • Epitopes
  • Colitis
  • Animals
  • Administration, Oral
 

Citation

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Chicago
ICMJE
MLA
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Curvino, E. J., Woodruff, M. E., Roe, E. F., Freire Haddad, H., Cordero Alvarado, P., & Collier, J. H. (2024). Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization. ACS Biomaterials Science & Engineering, 10(5), 3041–3056. https://doi.org/10.1021/acsbiomaterials.4c00525
Curvino, Elizabeth J., Mia E. Woodruff, Emily F. Roe, Helena Freire Haddad, Pablo Cordero Alvarado, and Joel H. Collier. “Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization.ACS Biomaterials Science & Engineering 10, no. 5 (May 2024): 3041–56. https://doi.org/10.1021/acsbiomaterials.4c00525.
Curvino EJ, Woodruff ME, Roe EF, Freire Haddad H, Cordero Alvarado P, Collier JH. Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization. ACS biomaterials science & engineering. 2024 May;10(5):3041–56.
Curvino, Elizabeth J., et al. “Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization.ACS Biomaterials Science & Engineering, vol. 10, no. 5, May 2024, pp. 3041–56. Epmc, doi:10.1021/acsbiomaterials.4c00525.
Curvino EJ, Woodruff ME, Roe EF, Freire Haddad H, Cordero Alvarado P, Collier JH. Supramolecular Peptide Self-Assemblies Facilitate Oral Immunization. ACS biomaterials science & engineering. 2024 May;10(5):3041–3056.
Journal cover image

Published In

ACS biomaterials science & engineering

DOI

EISSN

2373-9878

ISSN

2373-9878

Publication Date

May 2024

Volume

10

Issue

5

Start / End Page

3041 / 3056

Related Subject Headings

  • Peptides
  • Nanofibers
  • Mice, Inbred C57BL
  • Mice
  • Immunization
  • Female
  • Epitopes
  • Colitis
  • Animals
  • Administration, Oral