Skip to main content
Journal cover image

Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid.

Publication ,  Journal Article
Yu, G; Luo, T; Liu, Y; Huo, X; Mo, C; Huang, B; Li, Y; Feng, L; Sun, Y; Zhang, J; Zhang, Z
Published in: Ecotoxicol Environ Saf
June 15, 2024

Perfluorobutanesulfonic acid (PFBS), a short-chain alternative to perfluorooctanesulfonic acid (PFOS), is widely used in various products and is increasingly present in environmental media and human bodies. Recent epidemiological findings have raised concerns about its potential adverse health effects, although the specific toxic mechanism remains unclear. This study aimed to investigate the metabolic toxicity of gestational PFBS exposure in maternal rats. Pregnant Sprague Dawley (SD) rats were randomly assigned to three groups and administered either 3% starch gel (control), 5, or 50 mg/kg bw·d PFBS. Oral glucose tolerance tests (OGTT) and lipid profiles were measured, and integrated omics analysis (transcriptomics and non-targeted metabolomics) was employed to identify changes in genes and metabolites and their relationships with metabolic phenotypes. The results revealed that rats exposed to 50 mg/kg bw·d PFBS exhibited a significant decrease in 1-h glucose levels and the area under the curve (AUC) of OGTT compared with the starch group. Transcriptomics analysis indicated significant alterations in gene expression related to cytochrome P450 exogenous metabolism, glutathione metabolism, bile acid secretion, tumor pathways, and retinol metabolism. Differentially expressed metabolites (DEMs) were enriched in pathways such as pyruvate metabolism, the glucagon signaling pathway, central carbon metabolism in cancer, and the citric acid cycle. Co-enrichment analysis and pairwise correlation analysis among genes, metabolites, and outcomes identified several differentially expressed genes (DEGs), including Gstm1, Kit, Adcy1, Gck, Ppp1r3c, Ppp1r3d, and DEMs such as fumaric acid, L-lactic acid, 4-hydroxynonenal, and acetylvalerenolic acid. These DEGs and DEMs may play a role in the modulation of glucolipid metabolic pathways. In conclusion, our results suggest that gestational exposure to PFBS may induce molecular perturbations in glucose homeostasis. These findings provide insights into the potential mechanisms contributing to the heightened risk of abnormal glucose tolerance associated with PFBS exposure.

Duke Scholars

Published In

Ecotoxicol Environ Saf

DOI

EISSN

1090-2414

Publication Date

June 15, 2024

Volume

278

Start / End Page

116402

Location

Netherlands

Related Subject Headings

  • Sulfonic Acids
  • Strategic, Defence & Security Studies
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Multiomics
  • Metabolomics
  • Maternal Exposure
  • Homeostasis
  • Glucose Tolerance Test
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Yu, G., Luo, T., Liu, Y., Huo, X., Mo, C., Huang, B., … Zhang, Z. (2024). Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid. Ecotoxicol Environ Saf, 278, 116402. https://doi.org/10.1016/j.ecoenv.2024.116402
Yu, Guoqi, Tingyu Luo, Yongjie Liu, Xiaona Huo, Chunbao Mo, Bo Huang, You Li, et al. “Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid.Ecotoxicol Environ Saf 278 (June 15, 2024): 116402. https://doi.org/10.1016/j.ecoenv.2024.116402.
Yu G, Luo T, Liu Y, Huo X, Mo C, Huang B, et al. Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid. Ecotoxicol Environ Saf. 2024 Jun 15;278:116402.
Yu, Guoqi, et al. “Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid.Ecotoxicol Environ Saf, vol. 278, June 2024, p. 116402. Pubmed, doi:10.1016/j.ecoenv.2024.116402.
Yu G, Luo T, Liu Y, Huo X, Mo C, Huang B, Li Y, Feng L, Sun Y, Zhang J, Zhang Z. Multi-omics reveal disturbance of glucose homeostasis in pregnant rats exposed to short-chain perfluorobutanesulfonic acid. Ecotoxicol Environ Saf. 2024 Jun 15;278:116402.
Journal cover image

Published In

Ecotoxicol Environ Saf

DOI

EISSN

1090-2414

Publication Date

June 15, 2024

Volume

278

Start / End Page

116402

Location

Netherlands

Related Subject Headings

  • Sulfonic Acids
  • Strategic, Defence & Security Studies
  • Rats, Sprague-Dawley
  • Rats
  • Pregnancy
  • Multiomics
  • Metabolomics
  • Maternal Exposure
  • Homeostasis
  • Glucose Tolerance Test