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Identification and Characterization of miRNAs in Self-Rooted and Grafted Malus Reveals Critical Networks Associated with Flowering.

Publication ,  Journal Article
An, N; Fan, S; Yang, Y; Chen, X; Dong, F; Wang, Y; Xing, L; Zhao, C; Han, M
Published in: Int J Mol Sci
August 13, 2018

Grafting can improve the agricultural traits of crop plants, especially fruit trees. However, the regulatory networks and differentially expressed microRNAs (miRNAs) related to grafting in apple remain unclear. Herein, we conducted high-throughput sequencing and identified differentially expressed miRNAs among self-rooted Fuji, self-rooted M9, and grafted Fuji/M9. We analyzed the flowering rate, leaf morphology, and nutrient and carbohydrate contents in the three materials. The flowering rate, element and carbohydrate contents, and expression levels of flowering genes were higher in Fuji/M9 than in Fuji. We detected 206 known miRNAs and 976 novel miRNAs in the three materials, and identified those that were up- or downregulated in response to grafting. miR156 was most abundant in Fuji, followed by Fuji/M9, and then self-rooted M9, while miR172 was most abundant in M9, followed by Fuji/M9, and then Fuji. These expression patterns suggest that that these miRNAs were related to grafting. A Gene Ontology (GO) analysis showed that the differentially expressed miRNAs controlled genes involved in various biological processes, including cellular biosynthesis and metabolism. The expression of differentially expressed miRNAs and flowering-related genes was verified by qRT-PCR. Altogether, this comprehensive analysis of miRNAs related to grafting provides valuable information for breeding and grafting of apple and other fruit trees.

Duke Scholars

Published In

Int J Mol Sci

DOI

EISSN

1422-0067

Publication Date

August 13, 2018

Volume

19

Issue

8

Location

Switzerland

Related Subject Headings

  • Sugars
  • Sequence Analysis, RNA
  • RNA, Messenger
  • Plant Leaves
  • Phosphorus
  • Nitrogen
  • MicroRNAs
  • Malus
  • High-Throughput Nucleotide Sequencing
  • Gene Regulatory Networks
 

Citation

APA
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MLA
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An, N., Fan, S., Yang, Y., Chen, X., Dong, F., Wang, Y., … Han, M. (2018). Identification and Characterization of miRNAs in Self-Rooted and Grafted Malus Reveals Critical Networks Associated with Flowering. Int J Mol Sci, 19(8). https://doi.org/10.3390/ijms19082384
An, Na, Sheng Fan, Yang Yang, Xilong Chen, Feng Dong, Yibin Wang, Libo Xing, Caiping Zhao, and Mingyu Han. “Identification and Characterization of miRNAs in Self-Rooted and Grafted Malus Reveals Critical Networks Associated with Flowering.Int J Mol Sci 19, no. 8 (August 13, 2018). https://doi.org/10.3390/ijms19082384.
An, Na, et al. “Identification and Characterization of miRNAs in Self-Rooted and Grafted Malus Reveals Critical Networks Associated with Flowering.Int J Mol Sci, vol. 19, no. 8, Aug. 2018. Pubmed, doi:10.3390/ijms19082384.
An N, Fan S, Yang Y, Chen X, Dong F, Wang Y, Xing L, Zhao C, Han M. Identification and Characterization of miRNAs in Self-Rooted and Grafted Malus Reveals Critical Networks Associated with Flowering. Int J Mol Sci. 2018 Aug 13;19(8).

Published In

Int J Mol Sci

DOI

EISSN

1422-0067

Publication Date

August 13, 2018

Volume

19

Issue

8

Location

Switzerland

Related Subject Headings

  • Sugars
  • Sequence Analysis, RNA
  • RNA, Messenger
  • Plant Leaves
  • Phosphorus
  • Nitrogen
  • MicroRNAs
  • Malus
  • High-Throughput Nucleotide Sequencing
  • Gene Regulatory Networks