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Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems.

Publication ,  Journal Article
Li, J; Cupil-Garcia, V; Wang, H-N; Strobbia, P; Lai, B; Hu, J; Maiwald, M; Sumpf, B; Sun, T-P; Kemner, KM; Vo-Dinh, T
Published in: Biosensors & bioelectronics
October 2024

The intracellular developmental processes in plants, particularly concerning lignin polymer formation and biomass production are regulated by microRNAs (miRNAs). MiRNAs including miR397b are important for developing efficient and cost-effective biofuels. However, traditional methods of monitoring miRNA expression, like PCR, are time-consuming, require sample extraction, and lack spatial and temporal resolution, especially in real-world conditions. We present a novel approach using plasmonics nanosensing to monitor miRNA activity within living plant cells without sample extraction. Plasmonic biosensors using surface-enhanced Raman scattering (SERS) detection offer high sensitivity and precise molecular information. We used the Inverse Molecular Sentinel (iMS) biosensor on unique silver-coated gold nanorods (AuNR@Ag) with a high-aspect ratio to penetrate plant cell walls for detecting miR397b within intact living plant cells. MiR397b overexpression has shown promise in reducing lignin content. Thus, monitoring miR397b is essential for cost-effective biofuel generation. This study demonstrates the infiltration of nanorod iMS biosensors and detection of non-native miRNA 397b within plant cells for the first time. The investigation successfully demonstrates the localization of nanorod iMS biosensors through TEM and XRF-based elemental mapping for miRNA detection within plant cells of Nicotiana benthamiana. The study integrates shifted-excitation Raman difference spectroscopy (SERDS) to decrease background interference and enhance target signal extraction. In vivo SERDS testing confirms the dynamic detection of miR397b in Arabidopsis thaliana leaves after infiltration with iMS nanorods and miR397b target. This proof-of-concept study is an important stepping stone towards spatially resolved, intracellular miRNA mapping to monitor biomarkers and biological pathways for developing efficient renewable biofuel sources.

Duke Scholars

Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

October 2024

Volume

261

Start / End Page

116471

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver
  • Nicotiana
  • Nanotubes
  • MicroRNAs
  • Lignin
  • Gold
  • Biosensing Techniques
  • Biomarkers
  • Bioinformatics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Li, J., Cupil-Garcia, V., Wang, H.-N., Strobbia, P., Lai, B., Hu, J., … Vo-Dinh, T. (2024). Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems. Biosensors & Bioelectronics, 261, 116471. https://doi.org/10.1016/j.bios.2024.116471
Li, Joy, Vanessa Cupil-Garcia, Hsin-Neng Wang, Pietro Strobbia, Barry Lai, Jianhong Hu, Martin Maiwald, et al. “Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems.Biosensors & Bioelectronics 261 (October 2024): 116471. https://doi.org/10.1016/j.bios.2024.116471.
Li J, Cupil-Garcia V, Wang H-N, Strobbia P, Lai B, Hu J, et al. Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems. Biosensors & bioelectronics. 2024 Oct;261:116471.
Li, Joy, et al. “Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems.Biosensors & Bioelectronics, vol. 261, Oct. 2024, p. 116471. Epmc, doi:10.1016/j.bios.2024.116471.
Li J, Cupil-Garcia V, Wang H-N, Strobbia P, Lai B, Hu J, Maiwald M, Sumpf B, Sun T-P, Kemner KM, Vo-Dinh T. Plasmonics nanorod biosensor for in situ intracellular detection of gene expression biomarkers in intact plant systems. Biosensors & bioelectronics. 2024 Oct;261:116471.
Journal cover image

Published In

Biosensors & bioelectronics

DOI

EISSN

1873-4235

ISSN

0956-5663

Publication Date

October 2024

Volume

261

Start / End Page

116471

Related Subject Headings

  • Spectrum Analysis, Raman
  • Silver
  • Nicotiana
  • Nanotubes
  • MicroRNAs
  • Lignin
  • Gold
  • Biosensing Techniques
  • Biomarkers
  • Bioinformatics