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GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene.

Publication ,  Journal Article
Subramanyan, LV; Rasheed, SAK; Wang, L; Ghosh, S; Ong, MSN; Lakshmanan, M; Wang, M; Casey, PJ
Published in: Breast Cancer Res
July 4, 2024

GNA13 (Gα13) is one of two alpha subunit members of the G12/13 family of heterotrimeric G-proteins which mediate signaling downstream of GPCRs. It is known to be essential for embryonic development and vasculogenesis and has been increasingly shown to be involved in mediating several steps of cancer progression. Recent studies found that Gα13 can function as an oncogene and contributes to progression and metastasis of multiple tumor types, including ovarian, head and neck and prostate cancers. In most cases, Gα12 and Gα13, as closely related α-subunits in the subfamily, have similar cellular roles. However, in recent years their differences in signaling and function have started to emerge. We previously identified that Gα13 drives invasion of Triple Negative Breast Cancer (TNBC) cells in vitro. As a highly heterogenous disease with various well-defined molecular subtypes (ER+ /Her2-, ER+ /Her2+, Her2+, TNBC) and subtype associated outcomes, the function(s) of Gα13 beyond TNBC should be explored. Here, we report the finding that low expression of GNA13 is predictive of poorer survival in breast cancer, which challenges the conventional idea of Gα12/13 being universal oncogenes in solid tumors. Consistently, we found that Gα13 suppresses the proliferation in multiple ER+ breast cancer cell lines (MCF-7, ZR-75-1 and T47D). Loss of GNA13 expression drives cell proliferation, soft-agar colony formation and in vivo tumor formation in an orthotopic xenograft model. To evaluate the mechanism of Gα13 action, we performed RNA-sequencing analysis on these cell lines and found that loss of GNA13 results in the upregulation of MYC signaling pathways in ER+  breast cancer cells. Simultaneous silencing of MYC reversed the proliferative effect from the loss of GNA13, validating the role of MYC in Gα13 regulation of proliferation. Further, we found Gα13 regulates the expression of MYC, at both the transcript and protein level in an ERα dependent manner. Taken together, our study provides the first evidence for a tumor suppressive role for Gα13 in breast cancer cells and demonstrates for the first time the direct involvement of Gα13 in ER-dependent regulation of MYC signaling. With a few exceptions, elevated Gα13 levels are generally considered to be oncogenic, similar to Gα12. This study demonstrates an unexpected tumor suppressive role for Gα13 in ER+ breast cancer via regulation of MYC, suggesting that Gα13 can have subtype-dependent tumor suppressive roles in breast cancer.

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Published In

Breast Cancer Res

DOI

EISSN

1465-542X

Publication Date

July 4, 2024

Volume

26

Issue

1

Start / End Page

113

Location

England

Related Subject Headings

  • Up-Regulation
  • Triple Negative Breast Neoplasms
  • Signal Transduction
  • Proto-Oncogene Proteins c-myc
  • Oncology & Carcinogenesis
  • Mice
  • Humans
  • Gene Expression Regulation, Neoplastic
  • GTP-Binding Protein alpha Subunits, G12-G13
  • Female
 

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Subramanyan, L. V., Rasheed, S. A. K., Wang, L., Ghosh, S., Ong, M. S. N., Lakshmanan, M., … Casey, P. J. (2024). GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene. Breast Cancer Res, 26(1), 113. https://doi.org/10.1186/s13058-024-01866-x
Subramanyan, Lalitha Vaishnavi, Suhail Ahmed Kabeer Rasheed, Lijin Wang, Sujoy Ghosh, Michelle Shi Ning Ong, Manikandan Lakshmanan, Mei Wang, and Patrick J. Casey. “GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene.Breast Cancer Res 26, no. 1 (July 4, 2024): 113. https://doi.org/10.1186/s13058-024-01866-x.
Subramanyan LV, Rasheed SAK, Wang L, Ghosh S, Ong MSN, Lakshmanan M, et al. GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene. Breast Cancer Res. 2024 Jul 4;26(1):113.
Subramanyan, Lalitha Vaishnavi, et al. “GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene.Breast Cancer Res, vol. 26, no. 1, July 2024, p. 113. Pubmed, doi:10.1186/s13058-024-01866-x.
Subramanyan LV, Rasheed SAK, Wang L, Ghosh S, Ong MSN, Lakshmanan M, Wang M, Casey PJ. GNA13 suppresses proliferation of ER+ breast cancer cells via ERα dependent upregulation of the MYC oncogene. Breast Cancer Res. 2024 Jul 4;26(1):113.

Published In

Breast Cancer Res

DOI

EISSN

1465-542X

Publication Date

July 4, 2024

Volume

26

Issue

1

Start / End Page

113

Location

England

Related Subject Headings

  • Up-Regulation
  • Triple Negative Breast Neoplasms
  • Signal Transduction
  • Proto-Oncogene Proteins c-myc
  • Oncology & Carcinogenesis
  • Mice
  • Humans
  • Gene Expression Regulation, Neoplastic
  • GTP-Binding Protein alpha Subunits, G12-G13
  • Female