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An enhanced isothermal amplification assay for viral detection.

Publication ,  Journal Article
Qian, J; Boswell, SA; Chidley, C; Lu, Z-X; Pettit, ME; Gaudio, BL; Fajnzylber, JM; Ingram, RT; Ward, RH; Li, JZ; Springer, M
Published in: Nat Commun
November 20, 2020
Published version (DOI) Open Access Copy (Duke) Link to item

Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability.

Duke Scholars

Christopher Chidley
Author Christopher Chidley Pharmacology & Cancer Biology
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Published In

Nat Commun

DOI

10.1038/s41467-020-19258-y

EISSN

2041-1723

Publication Date

November 20, 2020

Volume

11

Issue

1

Start / End Page

5920

Location

England

Related Subject Headings

  • Virion
  • Saliva
  • SARS-CoV-2
  • Recombinases
  • Real-Time Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase
  • RNA, Viral
  • RNA
  • Nucleic Acid Amplification Techniques
  • Humans
 

Citation

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Qian, J., Boswell, S. A., Chidley, C., Lu, Z.-X., Pettit, M. E., Gaudio, B. L., … Springer, M. (2020). An enhanced isothermal amplification assay for viral detection. Nat Commun, 11(1), 5920. https://doi.org/10.1038/s41467-020-19258-y
Qian, Jason, Sarah A. Boswell, Christopher Chidley, Zhi-Xiang Lu, Mary E. Pettit, Benjamin L. Gaudio, Jesse M. Fajnzylber, et al. “An enhanced isothermal amplification assay for viral detection.” Nat Commun 11, no. 1 (November 20, 2020): 5920. https://doi.org/10.1038/s41467-020-19258-y.
Qian J, Boswell SA, Chidley C, Lu Z-X, Pettit ME, Gaudio BL, et al. An enhanced isothermal amplification assay for viral detection. Nat Commun. 2020 Nov 20;11(1):5920.
Qian, Jason, et al. “An enhanced isothermal amplification assay for viral detection.” Nat Commun, vol. 11, no. 1, Nov. 2020, p. 5920. Pubmed, doi:10.1038/s41467-020-19258-y.
Qian J, Boswell SA, Chidley C, Lu Z-X, Pettit ME, Gaudio BL, Fajnzylber JM, Ingram RT, Ward RH, Li JZ, Springer M. An enhanced isothermal amplification assay for viral detection. Nat Commun. 2020 Nov 20;11(1):5920.

Published In

Nat Commun

DOI

10.1038/s41467-020-19258-y

EISSN

2041-1723

Publication Date

November 20, 2020

Volume

11

Issue

1

Start / End Page

5920

Location

England

Related Subject Headings

  • Virion
  • Saliva
  • SARS-CoV-2
  • Recombinases
  • Real-Time Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase
  • RNA, Viral
  • RNA
  • Nucleic Acid Amplification Techniques
  • Humans