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Calcium channels from Cyprinus carpio skeletal muscle.

Publication ,  Journal Article
Grabner, M; Friedrich, K; Knaus, HG; Striessnig, J; Scheffauer, F; Staudinger, R; Koch, WJ; Schwartz, A; Glossmann, H
Published in: Proc Natl Acad Sci U S A
February 1, 1991
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The complete amino acid sequence of the L-type calcium channel alpha 1 subunit from the carp (Cyprinus carpio) white skeletal muscle was deduced by cDNA cloning and sequence analysis. The open reading frame encodes 1852 amino acids (Mr 210,060). A 155-amino acid COOH-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of L-type calcium channels. The photolabeled, membrane-bound, and purified carp alpha 1 subunits have masses of 211 and 190 kDa. The purified channel could not be phosphorylated by cAMP-dependent protein kinase. Two glycoproteins (alpha 2 subunits) are associated with the alpha 1 subunit and change their apparent masses from 235 and 220 kDa to 159 kDa upon reduction of disulfide bonds. Nucleic acid hybridization with alpha 2 cDNA revealed an 8.0-kilobase transcript in carp skeletal muscle. Evidence for a copurification of subunits similar in size to mammalian beta or gamma subunits was not obtained.

Duke Scholars

Walter J. Koch
Author Walter J. Koch Surgery, Cardiovascular and Thoracic Surgery

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.88.3.727

ISSN

0027-8424

Publication Date

February 1, 1991

Volume

88

Issue

3

Start / End Page

727 / 731

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Rabbits
  • Muscles
  • Molecular Sequence Data
  • Macromolecular Substances
  • Dihydropyridines
  • Carps
  • Calcium Channels
  • Biological Evolution
  • Azides
 

Citation

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MLA
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Grabner, M., Friedrich, K., Knaus, H. G., Striessnig, J., Scheffauer, F., Staudinger, R., … Glossmann, H. (1991). Calcium channels from Cyprinus carpio skeletal muscle. Proc Natl Acad Sci U S A, 88(3), 727–731. https://doi.org/10.1073/pnas.88.3.727
Grabner, M., K. Friedrich, H. G. Knaus, J. Striessnig, F. Scheffauer, R. Staudinger, W. J. Koch, A. Schwartz, and H. Glossmann. “Calcium channels from Cyprinus carpio skeletal muscle.” Proc Natl Acad Sci U S A 88, no. 3 (February 1, 1991): 727–31. https://doi.org/10.1073/pnas.88.3.727.
Grabner M, Friedrich K, Knaus HG, Striessnig J, Scheffauer F, Staudinger R, et al. Calcium channels from Cyprinus carpio skeletal muscle. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):727–31.
Grabner, M., et al. “Calcium channels from Cyprinus carpio skeletal muscle.” Proc Natl Acad Sci U S A, vol. 88, no. 3, Feb. 1991, pp. 727–31. Pubmed, doi:10.1073/pnas.88.3.727.
Grabner M, Friedrich K, Knaus HG, Striessnig J, Scheffauer F, Staudinger R, Koch WJ, Schwartz A, Glossmann H. Calcium channels from Cyprinus carpio skeletal muscle. Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):727–731.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.88.3.727

ISSN

0027-8424

Publication Date

February 1, 1991

Volume

88

Issue

3

Start / End Page

727 / 731

Location

United States

Related Subject Headings

  • Sequence Homology, Nucleic Acid
  • Rabbits
  • Muscles
  • Molecular Sequence Data
  • Macromolecular Substances
  • Dihydropyridines
  • Carps
  • Calcium Channels
  • Biological Evolution
  • Azides