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Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes.

Publication ,  Journal Article
Roe, EF; Freire Haddad, H; Lazar, KM; Liu, P; Collier, JH
Published in: Advanced healthcare materials
January 2025

Mucosal immune responses to vaccination are essential for achieving full protection against pathogens entering their host at mucosal sites. However, traditional parenteral immunization routes commonly fail to raise significant mucosal immunity. Sublingual immunization is a promising alternative delivery route to raise robust immune responses both systemically and at mucosal sites, and nanomaterial-based subunit vaccine platforms offer opportunities for raising epitope-specific responses. Here, sublingual immunization is reported using the Coil29 platform of coiled-coil self-assembling peptide nanofibers. The successful immunization with epitopes of varying physicochemical properties by including mucus-modulating components - namely sequences of proline, alanine, and serine (PAS) is demonstrated. PASylation is shown to decrease mucin complexation and increase epithelial penetration in vitro, enabling sublingual immunization against a variety of selected peptide epitopes in vivo. Coil29 fibers are also readily formed into tablets for solid-state dosing formulations and maintain their immunogenicity in this state. Previous sublingual peptide nanofiber immunotherapies have been based on different structures, such as highly stable β-sheets. The present work demonstrates that alternatively folded structures such as α-helical nanofibers can also be rendered sublingually immunogenic, enabling immunization with a variety of peptide epitopes and offering additional ways to specify mucus interactions, delivery state, dosing, and formulation.

Duke Scholars

Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

January 2025

Volume

14

Issue

3

Start / End Page

e2402055

Related Subject Headings

  • Vaccines
  • Peptides
  • Nanofibers
  • Mice, Inbred BALB C
  • Mice
  • Immunity, Mucosal
  • Humans
  • Female
  • Epitopes
  • Animals
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Roe, E. F., Freire Haddad, H., Lazar, K. M., Liu, P., & Collier, J. H. (2025). Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes. Advanced Healthcare Materials, 14(3), e2402055. https://doi.org/10.1002/adhm.202402055
Roe, Emily F., Helena Freire Haddad, Kat M. Lazar, Peiying Liu, and Joel H. Collier. “Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes.Advanced Healthcare Materials 14, no. 3 (January 2025): e2402055. https://doi.org/10.1002/adhm.202402055.
Roe EF, Freire Haddad H, Lazar KM, Liu P, Collier JH. Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes. Advanced healthcare materials. 2025 Jan;14(3):e2402055.
Roe, Emily F., et al. “Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes.Advanced Healthcare Materials, vol. 14, no. 3, Jan. 2025, p. e2402055. Epmc, doi:10.1002/adhm.202402055.
Roe EF, Freire Haddad H, Lazar KM, Liu P, Collier JH. Tuning Helical Peptide Nanofibers as a Sublingual Vaccine Platform for a Variety of Peptide Epitopes. Advanced healthcare materials. 2025 Jan;14(3):e2402055.
Journal cover image

Published In

Advanced healthcare materials

DOI

EISSN

2192-2659

ISSN

2192-2640

Publication Date

January 2025

Volume

14

Issue

3

Start / End Page

e2402055

Related Subject Headings

  • Vaccines
  • Peptides
  • Nanofibers
  • Mice, Inbred BALB C
  • Mice
  • Immunity, Mucosal
  • Humans
  • Female
  • Epitopes
  • Animals