Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples.
Publication
, Journal Article
Du, C; Volkan, P
Published in: Cold Spring Harbor protocols
January 2025
Chromatin immunoprecipitation (ChIP) is a common approach for studying the binding pattern of proteins on DNA sequences or the landscape of histones with different marks throughout the genome. ChIP is used on various organisms, including Drosophila This protocol provides a detailed overview of the immunoprecipitation portion of a ChIP procedure from samples of Drosophila nervous systems, specifically antennae and brains, that have already been fixed and sheared. These methods can be applied to other tissues of interest after optimizing for sample size and other relevant parameters.
Duke Scholars
Published In
Cold Spring Harbor protocols
DOI
EISSN
1559-6095
ISSN
1940-3402
Publication Date
January 2025
Volume
2025
Issue
1
Start / End Page
pdb.prot108142
Related Subject Headings
- Drosophila
- DNA
- Chromatin Immunoprecipitation
- Brain
- Arthropod Antennae
- Animals
- 3101 Biochemistry and cell biology
- 1103 Clinical Sciences
- 0601 Biochemistry and Cell Biology
- 0306 Physical Chemistry (incl. Structural)
Citation
APA
Chicago
ICMJE
MLA
NLM
Du, C., & Volkan, P. (2025). Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples. Cold Spring Harbor Protocols, 2025(1), pdb.prot108142. https://doi.org/10.1101/pdb.prot108142
Du, Chengcheng, and Pelin Volkan. “Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples.” Cold Spring Harbor Protocols 2025, no. 1 (January 2025): pdb.prot108142. https://doi.org/10.1101/pdb.prot108142.
Du C, Volkan P. Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples. Cold Spring Harbor protocols. 2025 Jan;2025(1):pdb.prot108142.
Du, Chengcheng, and Pelin Volkan. “Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples.” Cold Spring Harbor Protocols, vol. 2025, no. 1, Jan. 2025, p. pdb.prot108142. Epmc, doi:10.1101/pdb.prot108142.
Du C, Volkan P. Chromatin Immunoprecipitation (ChIP) Using Drosophila Antennal and Brain Samples. Cold Spring Harbor protocols. 2025 Jan;2025(1):pdb.prot108142.
Published In
Cold Spring Harbor protocols
DOI
EISSN
1559-6095
ISSN
1940-3402
Publication Date
January 2025
Volume
2025
Issue
1
Start / End Page
pdb.prot108142
Related Subject Headings
- Drosophila
- DNA
- Chromatin Immunoprecipitation
- Brain
- Arthropod Antennae
- Animals
- 3101 Biochemistry and cell biology
- 1103 Clinical Sciences
- 0601 Biochemistry and Cell Biology
- 0306 Physical Chemistry (incl. Structural)