Active transport enables protein condensation in cells.
Publication
, Journal Article
Chauhan, G; Wilkinson, EG; Yuan, Y; Cohen, SR; Onishi, M; Pappu, RV; Strader, LC
Published in: Science advances
May 2025
Multiple factors drive biomolecular condensate formation. In plants, condensation of the transcription factors AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19 attenuates response to the plant hormone auxin. Here, we report that actin-mediated movement of cytoplasmic ARF condensates enhances condensation. Coarse-grained molecular simulations of active polymers reveal that applied forces drive the associations of macromolecules to enhance phase separation while giving rise to dense phases that preferentially accumulate motile molecules. Our study highlights how molecular motility can drive phase separation, with implications for motile condensates while offering insights into cellular mechanisms that can regulate condensate dynamics.
Duke Scholars
Published In
Science advances
DOI
EISSN
2375-2548
ISSN
2375-2548
Publication Date
May 2025
Volume
11
Issue
21
Start / End Page
eadv7875
Related Subject Headings
- Transcription Factors
- Molecular Dynamics Simulation
- Indoleacetic Acids
- Biomolecular Condensates
- Biological Transport, Active
- Arabidopsis Proteins
- Arabidopsis
- Actins
Citation
APA
Chicago
ICMJE
MLA
NLM
Chauhan, G., Wilkinson, E. G., Yuan, Y., Cohen, S. R., Onishi, M., Pappu, R. V., & Strader, L. C. (2025). Active transport enables protein condensation in cells. Science Advances, 11(21), eadv7875. https://doi.org/10.1126/sciadv.adv7875
Chauhan, Gaurav, Edward G. Wilkinson, Yaning Yuan, Samuel R. Cohen, Masayuki Onishi, Rohit V. Pappu, and Lucia C. Strader. “Active transport enables protein condensation in cells.” Science Advances 11, no. 21 (May 2025): eadv7875. https://doi.org/10.1126/sciadv.adv7875.
Chauhan G, Wilkinson EG, Yuan Y, Cohen SR, Onishi M, Pappu RV, et al. Active transport enables protein condensation in cells. Science advances. 2025 May;11(21):eadv7875.
Chauhan, Gaurav, et al. “Active transport enables protein condensation in cells.” Science Advances, vol. 11, no. 21, May 2025, p. eadv7875. Epmc, doi:10.1126/sciadv.adv7875.
Chauhan G, Wilkinson EG, Yuan Y, Cohen SR, Onishi M, Pappu RV, Strader LC. Active transport enables protein condensation in cells. Science advances. 2025 May;11(21):eadv7875.
Published In
Science advances
DOI
EISSN
2375-2548
ISSN
2375-2548
Publication Date
May 2025
Volume
11
Issue
21
Start / End Page
eadv7875
Related Subject Headings
- Transcription Factors
- Molecular Dynamics Simulation
- Indoleacetic Acids
- Biomolecular Condensates
- Biological Transport, Active
- Arabidopsis Proteins
- Arabidopsis
- Actins