Effects of porcine hemagglutinating encephalomyelitis virus infection on astrocytes of mice
To clarify whether astrocytes are activated in the central nervous System of mice with PHEV infection,3-wcck-old BALB/c mice were inoculated with PHEV or PBS by intranasal route. GFAP was examined by using qRT-PCR, Western blot and indirect immunofluorescence (IF) at different time points aftcr PHEV infection.The results showed that the expression level of GFAP of mice infected with PHEV was significantly increased on the 3rd day aftcr inoculation with PHEV compared with control mice, and the expression level of GFAP increased gradually over time.The results of IF further confirmed the activation of astrocytes in PHEV infected mieewhich was characterized by increased cell number and concentrated distribution,as well as cell hypertro-phyand proliferation. These results indicated that astrocytes are activated in the brain of mice infected with PHEV.This study lays a foundation for the study of neuroinflammatory injury caused by PHEV infection.
