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The use of flow cytometry as a tool for monitoring filament formation of fungi.

Publication ,  Journal Article
Hopfer, RL; Sparks, SD; Cox, GM
Published in: Med Mycol
February 2001

Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.

Duke Scholars

Published In

Med Mycol

DOI

ISSN

1369-3786

Publication Date

February 2001

Volume

39

Issue

1

Start / End Page

103 / 107

Location

England

Related Subject Headings

  • Staining and Labeling
  • Reproducibility of Results
  • Microbiology
  • Flow Cytometry
  • Candida
  • 1108 Medical Microbiology
 

Citation

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Hopfer, R. L., Sparks, S. D., & Cox, G. M. (2001). The use of flow cytometry as a tool for monitoring filament formation of fungi. Med Mycol, 39(1), 103–107. https://doi.org/10.1080/mmy.39.1.103.107
Hopfer, R. L., S. D. Sparks, and G. M. Cox. “The use of flow cytometry as a tool for monitoring filament formation of fungi.Med Mycol 39, no. 1 (February 2001): 103–7. https://doi.org/10.1080/mmy.39.1.103.107.
Hopfer RL, Sparks SD, Cox GM. The use of flow cytometry as a tool for monitoring filament formation of fungi. Med Mycol. 2001 Feb;39(1):103–7.
Hopfer, R. L., et al. “The use of flow cytometry as a tool for monitoring filament formation of fungi.Med Mycol, vol. 39, no. 1, Feb. 2001, pp. 103–07. Pubmed, doi:10.1080/mmy.39.1.103.107.
Hopfer RL, Sparks SD, Cox GM. The use of flow cytometry as a tool for monitoring filament formation of fungi. Med Mycol. 2001 Feb;39(1):103–107.
Journal cover image

Published In

Med Mycol

DOI

ISSN

1369-3786

Publication Date

February 2001

Volume

39

Issue

1

Start / End Page

103 / 107

Location

England

Related Subject Headings

  • Staining and Labeling
  • Reproducibility of Results
  • Microbiology
  • Flow Cytometry
  • Candida
  • 1108 Medical Microbiology