
The use of flow cytometry as a tool for monitoring filament formation of fungi.
Flow cytometry (FC) has the ability to discriminate a variety of cell parameters including cell size and complexity, and fluorescence intensity. As yeast cells or fungal spores germinate they undergo a morphological transformation from round oval shaped cells to elongate filamentous forms. To date, monitoring these events has been performed using microscopic examination. Microscopic examination is a labor intensive process that examines a very small percentage of the total cell population. We have developed a method using FC that is rapid, simple to perform, and reproducible. The major advantages of FC include analysis of a larger number of cells, increased objectivity due to nonselective measurements of all cells in the population studied, and the computer related data analysis capability of the flow cytometer.
Duke Scholars
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- Staining and Labeling
- Reproducibility of Results
- Microbiology
- Flow Cytometry
- Candida
- 1108 Medical Microbiology
Citation

Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Staining and Labeling
- Reproducibility of Results
- Microbiology
- Flow Cytometry
- Candida
- 1108 Medical Microbiology