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Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.

Publication ,  Journal Article
Mason, IJ; Taylor, A; Williams, JG; Sage, H; Hogan, BL
Published in: EMBO J
July 1986

We describe the molecular cloning and characterization of a secreted, acidic, cysteine-rich glycoprotein (SPARC) of apparent Mr 43,000 which is a major product of mouse embryo parietal endoderm. These cells are specialized for the synthesis of a rapidly expanding basement membrane, but SPARC is not itself an integral matrix component. We show that SPARC is related structurally and antigenically to an Mr 43,000 glycoprotein secreted in large amounts by bovine aortic endothelial cells as part of a 'culture shock' response to in vitro conditions promoting their proliferation and migration.

Duke Scholars

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

July 1986

Volume

5

Issue

7

Start / End Page

1465 / 1472

Location

England

Related Subject Headings

  • Tumor Suppressor Proteins
  • Transcription, Genetic
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Osteonectin
  • Molecular Weight
  • Molecular Sequence Data
  • Mice
  • Glycoproteins
  • Endothelium
 

Citation

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Mason, I. J., Taylor, A., Williams, J. G., Sage, H., & Hogan, B. L. (1986). Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000. EMBO J, 5(7), 1465–1472. https://doi.org/10.1002/j.1460-2075.1986.tb04383.x
Mason, I. J., A. Taylor, J. G. Williams, H. Sage, and B. L. Hogan. “Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.EMBO J 5, no. 7 (July 1986): 1465–72. https://doi.org/10.1002/j.1460-2075.1986.tb04383.x.
Mason, I. J., et al. “Evidence from molecular cloning that SPARC, a major product of mouse embryo parietal endoderm, is related to an endothelial cell 'culture shock' glycoprotein of Mr 43,000.EMBO J, vol. 5, no. 7, July 1986, pp. 1465–72. Pubmed, doi:10.1002/j.1460-2075.1986.tb04383.x.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

July 1986

Volume

5

Issue

7

Start / End Page

1465 / 1472

Location

England

Related Subject Headings

  • Tumor Suppressor Proteins
  • Transcription, Genetic
  • Species Specificity
  • Sequence Homology, Nucleic Acid
  • Osteonectin
  • Molecular Weight
  • Molecular Sequence Data
  • Mice
  • Glycoproteins
  • Endothelium