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Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines.

Publication ,  Journal Article
Labosky, PA; Barlow, DP; Hogan, BL
Published in: Development
November 1994

Primordial germ cells of the mouse cultured on feeder layers with leukemia inhibitory factor, Steel factor and basic fibroblast growth factor give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells. These primordial germ cell-derived embryonic germ cells can be induced to differentiate extensively in culture, form teratocarcinomas when injected into nude mice and contribute to chimeras when injected into host blastocysts. Here, we report the derivation of multiple embryonic germ cell lines from 8.5 days post coitum embryos of C57BL/6 inbred mice. Four independent embryonic germ cell lines with normal male karyotypes have formed chimeras when injected into BALB/c host blastocysts and two of these lines have transmitted coat color markers through the germline. We also show that pluripotent cell lines capable of forming teratocarcinomas and coat color chimeras can be established from primordial germ cells of 8.0 days p.c. embryos and 12.5 days p.c. genital ridges. We have examined the methylation status of the putative imprinting box of the insulin-like growth factor type 2 receptor gene (Igf2r) in these embryonic germ cell lines. No correlation was found between methylation pattern and germline competence. A significant difference was observed between embryonic stem cell and embryonic germ cell lines in their ability to maintain the methylation imprint of the Igf2r gene in culture. This may illustrate a fundamental difference between these two cell types.

Duke Scholars

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Published In

Development

DOI

ISSN

0950-1991

Publication Date

November 1994

Volume

120

Issue

11

Start / End Page

3197 / 3204

Location

England

Related Subject Headings

  • Stem Cells
  • Receptor, IGF Type 2
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice, Inbred Strains
  • Mice
  • Methylation
  • Germ Cells
  • Genomic Imprinting
 

Citation

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MLA
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Labosky, P. A., D. P. Barlow, and B. L. Hogan. “Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines.Development 120, no. 11 (November 1994): 3197–3204. https://doi.org/10.1242/dev.120.11.3197.
Journal cover image

Published In

Development

DOI

ISSN

0950-1991

Publication Date

November 1994

Volume

120

Issue

11

Start / End Page

3197 / 3204

Location

England

Related Subject Headings

  • Stem Cells
  • Receptor, IGF Type 2
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice, Inbred Strains
  • Mice
  • Methylation
  • Germ Cells
  • Genomic Imprinting