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The intragraft CD8+ T cell response in renal allograft rejection in the mouse.

Publication ,  Journal Article
Mannon, RB; Kotzin, BL; Roper, E; Nataraj, C; Kurlander, RJ; Coffman, TM
Published in: Transplantation
July 15, 1996

To identify the role of donor class I alloantigens in regulating the CD8+ T cell response to a kidney allograft, we analyzed and compared the CD8+ infiltrate in kidney transplants from MHC class I-deficient (class I-) mouse donors and class I+ controls. One week after transplantation, there was a prominent CD8+ infiltrate in control allografts, whereas CD8+ T cells were virtually absent in grafts from class I- donors. In class I+ allografts, infiltrating CD8+ cells utilized a wide range of T cell receptor (TCR) Vbeta families and their Vbeta usage was similar to that of the systemic CD8+ population. However, there was a modest but significant overrepresentation of cells bearing Vbeta8 in the graft compared with the spleen due to an expansion of CD8+ Vbeta8.3+ cells. This could be detected as early as 1 week and became more pronounced by 3 weeks after transplantation. In 3-week allografts, only 52% of CD8+ cells expressed alphabetaTCR. Among T cells isolated from class I+ grafts, the CD8+ Vbeta8+ cells demonstrated allospecific responses that were numerically larger than responses of the CD8+ Vbeta8- population. In contrast to the early (1 week) time point, significant numbers of CD8+ cells could be isolated from class I- grafts by 3 weeks after transplantation and their Vbeta repertoire resembled that seen in controls. While increasing numbers of CD8+ Vbeta8+ were present in the class I- grafts at 3 weeks, this increase was not statistically significant. Thus, expression of class I alloantigens on a kidney graft plays an important role in regulating the rate of accumulation of CD8+ T cells in rejecting kidney grafts. However, the TCR Vbeta repertoire of the CD8+ T cell infiltrate is largely determined by factors that are independent of normal class I expression on the graft.

Duke Scholars

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

July 15, 1996

Volume

62

Issue

1

Start / End Page

96 / 104

Location

United States

Related Subject Headings

  • Surgery
  • Spleen
  • Receptors, Antigen, T-Cell, alpha-beta
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Count
  • Kidney Transplantation
  • Interferon-gamma
  • Graft Rejection
  • Flow Cytometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Mannon, R. B., Kotzin, B. L., Roper, E., Nataraj, C., Kurlander, R. J., & Coffman, T. M. (1996). The intragraft CD8+ T cell response in renal allograft rejection in the mouse. Transplantation, 62(1), 96–104. https://doi.org/10.1097/00007890-199607150-00019
Mannon, R. B., B. L. Kotzin, E. Roper, C. Nataraj, R. J. Kurlander, and T. M. Coffman. “The intragraft CD8+ T cell response in renal allograft rejection in the mouse.Transplantation 62, no. 1 (July 15, 1996): 96–104. https://doi.org/10.1097/00007890-199607150-00019.
Mannon RB, Kotzin BL, Roper E, Nataraj C, Kurlander RJ, Coffman TM. The intragraft CD8+ T cell response in renal allograft rejection in the mouse. Transplantation. 1996 Jul 15;62(1):96–104.
Mannon, R. B., et al. “The intragraft CD8+ T cell response in renal allograft rejection in the mouse.Transplantation, vol. 62, no. 1, July 1996, pp. 96–104. Pubmed, doi:10.1097/00007890-199607150-00019.
Mannon RB, Kotzin BL, Roper E, Nataraj C, Kurlander RJ, Coffman TM. The intragraft CD8+ T cell response in renal allograft rejection in the mouse. Transplantation. 1996 Jul 15;62(1):96–104.

Published In

Transplantation

DOI

ISSN

0041-1337

Publication Date

July 15, 1996

Volume

62

Issue

1

Start / End Page

96 / 104

Location

United States

Related Subject Headings

  • Surgery
  • Spleen
  • Receptors, Antigen, T-Cell, alpha-beta
  • Mice, Inbred C57BL
  • Mice
  • Lymphocyte Count
  • Kidney Transplantation
  • Interferon-gamma
  • Graft Rejection
  • Flow Cytometry