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Gene targeting: applications in transplantation research.

Publication ,  Journal Article
Mannon, RB; Coffman, TM
Published in: Kidney Int
July 1999

Gene targeting, the manipulation of gene in the mouse genome using homologous recombination in embryonic stem cells, is a powerful experimental tool that has been widely utilized in a number of disciplines. The ability to precisely alter genes in this way provides an avenue for investigating the role of a gene product in normal and pathological processes in the intact animal, with a precision and efficacy not possible using pharmacological agents, antibodies or engineered proteins. In transplant research, gene targeting provides a unique tool for discriminating the contributions of gene expression in donor versus recipient tissues. This review focuses on several areas in transplantation research where gene targeting has made useful contributions. These include studies of the role of donor and recipient multiple histocompatibility complex antigens in regulating rejection responses, the role of CD4+ T cell in mediating acute rejection, and the functions of cytokines during rejection and tolerance induction. These studies highlight the unique advantages of gene targeting in studies of complex processes in whole animals and illustrate the contributions of this technique to understanding the pathogenesis of allograft rejection.

Duke Scholars

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

July 1999

Volume

56

Issue

1

Start / End Page

18 / 27

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Transplantation
  • Research
  • Humans
  • Histocompatibility Antigens
  • Graft Rejection
  • Gene Targeting
  • Cytokines
  • CD8-Positive T-Lymphocytes
  • CD4-Positive T-Lymphocytes
 

Citation

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MLA
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Mannon, R. B., & Coffman, T. M. (1999). Gene targeting: applications in transplantation research. Kidney Int, 56(1), 18–27. https://doi.org/10.1046/j.1523-1755.1999.00513.x
Mannon, R. B., and T. M. Coffman. “Gene targeting: applications in transplantation research.Kidney Int 56, no. 1 (July 1999): 18–27. https://doi.org/10.1046/j.1523-1755.1999.00513.x.
Mannon RB, Coffman TM. Gene targeting: applications in transplantation research. Kidney Int. 1999 Jul;56(1):18–27.
Mannon, R. B., and T. M. Coffman. “Gene targeting: applications in transplantation research.Kidney Int, vol. 56, no. 1, July 1999, pp. 18–27. Pubmed, doi:10.1046/j.1523-1755.1999.00513.x.
Mannon RB, Coffman TM. Gene targeting: applications in transplantation research. Kidney Int. 1999 Jul;56(1):18–27.
Journal cover image

Published In

Kidney Int

DOI

ISSN

0085-2538

Publication Date

July 1999

Volume

56

Issue

1

Start / End Page

18 / 27

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Transplantation
  • Research
  • Humans
  • Histocompatibility Antigens
  • Graft Rejection
  • Gene Targeting
  • Cytokines
  • CD8-Positive T-Lymphocytes
  • CD4-Positive T-Lymphocytes