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Effect of fatty acids on insulin release: role of chain length and degree of unsaturation.

Publication ,  Journal Article
Opara, EC; Garfinkel, M; Hubbard, VS; Burch, WM; Akwari, OE
Published in: Am J Physiol
April 1994

The purpose of the present study was to examine the role played by structural differences among fatty acids in their effect on insulin secretion by isolated perifused murine islets. Insulin secretion measured by radioimmunoassay was assessed either as total insulin output (ng.6 islets-1.20 min-1) or as percent of basal insulin secretion. Raising the glucose concentration from a basal 5.5 to 27.7 mM caused an increase of insulin output from 6.69 +/- 1.59 to 19.92 +/- 4.99 ng.6 islets-1.20 min-1 (P < 0.05) in control (untreated) islets. However, after 20-min exposure of islets to 5 mM 16:0 or 18:2, the effect of 27.7 mM glucose was enhanced or diminished, respectively. Basal insulin output (100% basal) changed to 44 +/- 10% basal (P < 0.005) with the addition of 5 mM 4:0 but was not altered when 4:0 was replaced by 6:0. Insulin output increased modestly with 5 mM 8:0 but significantly (P < 0.05) with 10:0 until a maximal of 280 +/- 24% basal with 12:0 (P < 0.01), then fell to 110 +/- 18 and 93 +/- 15% basal (P < 0.05) with 14:0 and 16:0, respectively. The addition of 5 mM 18:0 inhibited insulin secretion to 30 +/- 10% of basal (P < 0.003), and this effect was not caused by fatty acid interference with insulin assay.(ABSTRACT TRUNCATED AT 250 WORDS)

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1994

Volume

266

Issue

4 Pt 1

Start / End Page

E635 / E639

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Radioimmunoassay
  • Mice, Inbred Strains
  • Mice
  • Islets of Langerhans
  • Insulin Secretion
  • Insulin
  • In Vitro Techniques
  • Glucose
  • Female
 

Citation

APA
Chicago
ICMJE
MLA
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Opara, E. C., Garfinkel, M., Hubbard, V. S., Burch, W. M., & Akwari, O. E. (1994). Effect of fatty acids on insulin release: role of chain length and degree of unsaturation. Am J Physiol, 266(4 Pt 1), E635–E639. https://doi.org/10.1152/ajpendo.1994.266.4.E635
Opara, E. C., M. Garfinkel, V. S. Hubbard, W. M. Burch, and O. E. Akwari. “Effect of fatty acids on insulin release: role of chain length and degree of unsaturation.Am J Physiol 266, no. 4 Pt 1 (April 1994): E635–39. https://doi.org/10.1152/ajpendo.1994.266.4.E635.
Opara EC, Garfinkel M, Hubbard VS, Burch WM, Akwari OE. Effect of fatty acids on insulin release: role of chain length and degree of unsaturation. Am J Physiol. 1994 Apr;266(4 Pt 1):E635–9.
Opara, E. C., et al. “Effect of fatty acids on insulin release: role of chain length and degree of unsaturation.Am J Physiol, vol. 266, no. 4 Pt 1, Apr. 1994, pp. E635–39. Pubmed, doi:10.1152/ajpendo.1994.266.4.E635.
Opara EC, Garfinkel M, Hubbard VS, Burch WM, Akwari OE. Effect of fatty acids on insulin release: role of chain length and degree of unsaturation. Am J Physiol. 1994 Apr;266(4 Pt 1):E635–E639.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

April 1994

Volume

266

Issue

4 Pt 1

Start / End Page

E635 / E639

Location

United States

Related Subject Headings

  • Structure-Activity Relationship
  • Radioimmunoassay
  • Mice, Inbred Strains
  • Mice
  • Islets of Langerhans
  • Insulin Secretion
  • Insulin
  • In Vitro Techniques
  • Glucose
  • Female