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Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study.

Publication ,  Journal Article
Helm, RM; Buckley, RH; Adkinson, NF; Squillace, DL; Gleich, GJ; Yunginger, JW
Published in: J Allergy Clin Immunol
June 1986

The sensitivity, specificity, and precision of immunoassays for quantitation of IgE in cell-culture supernatants were tested in a multicenter trial involving 22 laboratories. Fourteen coded test samples included cell-culture medium alone, culture medium with varying concentrations of polyclonal or myeloma IgE, and medium from unstimulated or pokeweed mitogen (PWM)-stimulated peripheral blood mononuclear cell (PBMC)-culture supernatants. Two laboratories reported measurable IgE in non-IgE-containing control samples. Although the IgE content of a single 0.50 ng/ml polyclonal IgE sample should have been measured easily by the claims of all laboratories, only 13 laboratories measured IgE in this sample in 22 of the 48 assays. Most laboratories could measure both polyclonal and myeloma IgE at 5.0 ng/ml; however, the IgE determinations for the myeloma proteins were nearer the predicted value. Although 15 laboratories found measurable IgE in the PWM-stimulated PBMC-culture supernatants from a single nonatopic donor, the levels did not differ significantly from that measured in unstimulated PBMC-culture supernatants. Three laboratories reported considerably higher IgE levels in the PWM-stimulated PBMC-culture supernatants than in other PBMC-culture supernatants. Only 13 laboratories could quantitate IgE in each of coded duplicate samples containing 0.50 ng/ml polyclonal IgE. These findings indicate a wide variability in the sensitivity, specificity, and precision of assays used to quantitate low levels of IgE protein. Investigators should be encouraged to make greater use of existing national and international reference materials in the standardization and performance of their IgE immunoassays.

Duke Scholars

Published In

J Allergy Clin Immunol

DOI

ISSN

0091-6749

Publication Date

June 1986

Volume

77

Issue

6

Start / End Page

880 / 890

Location

United States

Related Subject Headings

  • Immunoglobulin E
  • Humans
  • Double-Blind Method
  • Allergy
  • 3204 Immunology
  • 1107 Immunology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Helm, R. M., Buckley, R. H., Adkinson, N. F., Squillace, D. L., Gleich, G. J., & Yunginger, J. W. (1986). Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study. J Allergy Clin Immunol, 77(6), 880–890. https://doi.org/10.1016/0091-6749(86)90387-8
Helm, R. M., R. H. Buckley, N. F. Adkinson, D. L. Squillace, G. J. Gleich, and J. W. Yunginger. “Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study.J Allergy Clin Immunol 77, no. 6 (June 1986): 880–90. https://doi.org/10.1016/0091-6749(86)90387-8.
Helm RM, Buckley RH, Adkinson NF, Squillace DL, Gleich GJ, Yunginger JW. Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study. J Allergy Clin Immunol. 1986 Jun;77(6):880–90.
Helm, R. M., et al. “Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study.J Allergy Clin Immunol, vol. 77, no. 6, June 1986, pp. 880–90. Pubmed, doi:10.1016/0091-6749(86)90387-8.
Helm RM, Buckley RH, Adkinson NF, Squillace DL, Gleich GJ, Yunginger JW. Variability of IgE protein measurement in cell-culture supernatants: results from a multicenter collaborative study. J Allergy Clin Immunol. 1986 Jun;77(6):880–890.
Journal cover image

Published In

J Allergy Clin Immunol

DOI

ISSN

0091-6749

Publication Date

June 1986

Volume

77

Issue

6

Start / End Page

880 / 890

Location

United States

Related Subject Headings

  • Immunoglobulin E
  • Humans
  • Double-Blind Method
  • Allergy
  • 3204 Immunology
  • 1107 Immunology