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In vitro studies of IgE synthesis by human blood mononuclear cells.

Publication ,  Journal Article
Buckley, RH; Fiser, PM; Becker, WG
Published in: Fed Proc
June 1981

IgE was detected in increasing quantities in supernatants of cultured human blood mononuclear cells, with peak amounts being found in 7 to 12-day cultures. Inhibitors of protein synthesis or of cell division reduced IgE production. Significantly greater amounts of IgE were made by cells from patients with elevated serum IgE than by those from normal adults. Pokeweed mitogen caused modest (threefold) augmentation of IgE synthesis is normal control cultures but inhibited it in cultures from patients with elevated serum IgE. Co-culturing unfractionated mononuclear cells from some atopic patients with cells from normal people resulted in significant suppression of the expected amount of IgE synthesis but not of IgM or IgG production. This suggests that a suppressor cell population or function is present in normal adults that regulates B cell IgE synthesis and that patients with elevated IgE may be deficient in this function. Results of coculture studies with isolated T and B cell-enriched subpopulations support the possibility that such activity exists in normal T cell populations. The marked augmentation of IgE synthesis by patients' B cells when separated from their own T cells indicates that patients' T cells have some regulatory capacity, but control T cells were more potent in this regard.

Duke Scholars

Published In

Fed Proc

ISSN

0014-9446

Publication Date

June 1981

Volume

40

Issue

8

Start / End Page

2167 / 2170

Location

United States

Related Subject Headings

  • Reference Values
  • Receptors, Fc
  • Pokeweed Mitogens
  • Monocytes
  • Immunoglobulin E
  • Immune System Diseases
  • Humans
  • Eczema
  • Cells, Cultured
  • Biochemistry & Molecular Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Buckley, R. H., Fiser, P. M., & Becker, W. G. (1981). In vitro studies of IgE synthesis by human blood mononuclear cells. Fed Proc, 40(8), 2167–2170.
Buckley, R. H., P. M. Fiser, and W. G. Becker. “In vitro studies of IgE synthesis by human blood mononuclear cells.Fed Proc 40, no. 8 (June 1981): 2167–70.
Buckley RH, Fiser PM, Becker WG. In vitro studies of IgE synthesis by human blood mononuclear cells. Fed Proc. 1981 Jun;40(8):2167–70.
Buckley, R. H., et al. “In vitro studies of IgE synthesis by human blood mononuclear cells.Fed Proc, vol. 40, no. 8, June 1981, pp. 2167–70.
Buckley RH, Fiser PM, Becker WG. In vitro studies of IgE synthesis by human blood mononuclear cells. Fed Proc. 1981 Jun;40(8):2167–2170.

Published In

Fed Proc

ISSN

0014-9446

Publication Date

June 1981

Volume

40

Issue

8

Start / End Page

2167 / 2170

Location

United States

Related Subject Headings

  • Reference Values
  • Receptors, Fc
  • Pokeweed Mitogens
  • Monocytes
  • Immunoglobulin E
  • Immune System Diseases
  • Humans
  • Eczema
  • Cells, Cultured
  • Biochemistry & Molecular Biology