Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel

Intravitreal clearance of tissue plasminogen activator in the rabbit.

Publication ,  Journal Article
Jaffe, GJ; Green, GD; McKay, BS; Hartz, A; Williams, GA
Published in: Arch Ophthalmol
July 1988

The clearance of tissue plasminogen activator (t-PA) injected into the midvitreous cavity was studied in the phakic, vitrectomized rabbit eye with and without intravitreal fibrin clots. The quantity and activity of t-PA in the vitreous, serum, and aqueous were determined at ten minutes and at 3, 6, 15, 24, and 48 hours after initial injection by an enzyme-linked immunosorbent assay (ELISA) and a spectrophotometric solid-phase fibrin assay (SOFIA). In eyes without an intravitreal fibrin clot, the estimated half-life for t-PA was 4.3 hours by SOFIA and 5.8 hours by ELISA. In eyes containing a vitreal fibrin clot, the half-life increased to 9.8 hours by SOFIA and 11.9 hours by ELISA. Both of these half-lives were significantly greater than the half-life for eyes without fibrin. Regardless of the presence of fibrin, intravitreal t-PA activity was significantly less than t-PA quantity, suggesting the presence of a t-PA inhibitor. A peak in aqueous t-PA occurred before six hours, indicating that t-PA was cleared in part through the anterior chamber. There was no measurable serum t-PA at any of the sampling times.

Duke Scholars

Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

July 1988

Volume

106

Issue

7

Start / End Page

969 / 972

Location

United States

Related Subject Headings

  • Vitreous Body
  • Tissue Plasminogen Activator
  • Spectrophotometry
  • Rabbits
  • Ophthalmology & Optometry
  • Fibrin
  • Enzyme-Linked Immunosorbent Assay
  • Aqueous Humor
  • Animals
  • 1113 Opthalmology and Optometry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Jaffe, G. J., Green, G. D., McKay, B. S., Hartz, A., & Williams, G. A. (1988). Intravitreal clearance of tissue plasminogen activator in the rabbit. Arch Ophthalmol, 106(7), 969–972. https://doi.org/10.1001/archopht.1988.01060140115036
Jaffe, G. J., G. D. Green, B. S. McKay, A. Hartz, and G. A. Williams. “Intravitreal clearance of tissue plasminogen activator in the rabbit.Arch Ophthalmol 106, no. 7 (July 1988): 969–72. https://doi.org/10.1001/archopht.1988.01060140115036.
Jaffe GJ, Green GD, McKay BS, Hartz A, Williams GA. Intravitreal clearance of tissue plasminogen activator in the rabbit. Arch Ophthalmol. 1988 Jul;106(7):969–72.
Jaffe, G. J., et al. “Intravitreal clearance of tissue plasminogen activator in the rabbit.Arch Ophthalmol, vol. 106, no. 7, July 1988, pp. 969–72. Pubmed, doi:10.1001/archopht.1988.01060140115036.
Jaffe GJ, Green GD, McKay BS, Hartz A, Williams GA. Intravitreal clearance of tissue plasminogen activator in the rabbit. Arch Ophthalmol. 1988 Jul;106(7):969–972.

Published In

Arch Ophthalmol

DOI

ISSN

0003-9950

Publication Date

July 1988

Volume

106

Issue

7

Start / End Page

969 / 972

Location

United States

Related Subject Headings

  • Vitreous Body
  • Tissue Plasminogen Activator
  • Spectrophotometry
  • Rabbits
  • Ophthalmology & Optometry
  • Fibrin
  • Enzyme-Linked Immunosorbent Assay
  • Aqueous Humor
  • Animals
  • 1113 Opthalmology and Optometry