Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.
Publication
, Journal Article
Moore, PM; Hayes, EC; Miller, SE; Wright, LL; Machamer, CE; Zweerink, HJ
Published in: Infect Immun
June 1978
Nucleocapsids in quantities approaching 1 mg were purified from 109 measles virus-infected cells. They contained one polypeptide species with a molecular weight of 59,000. Antiserum was raised in rabbits against purified nucleocapsids and used in a competitive radioimmunoassay. Because of their instability, purified nucleocapsids were not suitable for use in such an assay. Instead partially purified nucleocapsids from HEp-2 cells persistently infected with measles virus and labeled in vitro with [35S]methionine were used as the source of radioactive antigen. The radioimmunoassay thus developed measured less than 5 ng of nucleocapsids in infected cells.
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Published In
Infect Immun
DOI
ISSN
0019-9567
Publication Date
June 1978
Volume
20
Issue
3
Start / End Page
842 / 846
Location
United States
Related Subject Headings
- Viral Proteins
- Radioimmunoassay
- Rabbits
- RNA, Viral
- Microbiology
- Measles virus
- Immunosorbent Techniques
- Electrophoresis, Polyacrylamide Gel
- Cell Line
- Capsid
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Chicago
ICMJE
MLA
NLM
Moore, P. M., Hayes, E. C., Miller, S. E., Wright, L. L., Machamer, C. E., & Zweerink, H. J. (1978). Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. Infect Immun, 20(3), 842–846. https://doi.org/10.1128/iai.20.3.842-846.1978
Moore, P. M., E. C. Hayes, S. E. Miller, L. L. Wright, C. E. Machamer, and H. J. Zweerink. “Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.” Infect Immun 20, no. 3 (June 1978): 842–46. https://doi.org/10.1128/iai.20.3.842-846.1978.
Moore PM, Hayes EC, Miller SE, Wright LL, Machamer CE, Zweerink HJ. Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. Infect Immun. 1978 Jun;20(3):842–6.
Moore, P. M., et al. “Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays.” Infect Immun, vol. 20, no. 3, June 1978, pp. 842–46. Pubmed, doi:10.1128/iai.20.3.842-846.1978.
Moore PM, Hayes EC, Miller SE, Wright LL, Machamer CE, Zweerink HJ. Measles virus nucleocapsids: large-scale purification and use in radioimmunoassays. Infect Immun. 1978 Jun;20(3):842–846.
Published In
Infect Immun
DOI
ISSN
0019-9567
Publication Date
June 1978
Volume
20
Issue
3
Start / End Page
842 / 846
Location
United States
Related Subject Headings
- Viral Proteins
- Radioimmunoassay
- Rabbits
- RNA, Viral
- Microbiology
- Measles virus
- Immunosorbent Techniques
- Electrophoresis, Polyacrylamide Gel
- Cell Line
- Capsid