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Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis.

Publication ,  Journal Article
Silverstone, AL; Jung, HS; Dill, A; Kawaide, H; Kamiya, Y; Sun, TP
Published in: The Plant cell
July 2001

RGA (for repressor of ga1-3) and SPINDLY (SPY) are likely repressors of gibberellin (GA) signaling in Arabidopsis because the recessive rga and spy mutations partially suppressed the phenotype of the GA-deficient mutant ga1-3. We found that neither rga nor spy altered the GA levels in the wild-type or the ga1-3 background. However, expression of the GA biosynthetic gene GA4 was reduced 26% by the rga mutation, suggesting that partial derepression of the GA response pathway by rga resulted in the feedback inhibition of GA4 expression. The green fluorescent protein (GFP)-RGA fusion protein was localized to nuclei in transgenic Arabidopsis. This result supports the predicted function of RGA as a transcriptional regulator based on sequence analysis. Confocal microscopy and immunoblot analyses demonstrated that the levels of both the GFP-RGA fusion protein and endogenous RGA were reduced rapidly by GA treatment. Therefore, the GA signal appears to derepress the GA signaling pathway by degrading the repressor protein RGA. The effect of rga on GA4 gene expression and the effect of GA on RGA protein level allow us to identify part of the mechanism by which GA homeostasis is achieved.

Duke Scholars

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2001

Volume

13

Issue

7

Start / End Page

1555 / 1566

Related Subject Headings

  • Transcription Factors
  • Suppression, Genetic
  • Signal Transduction
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • RNA, Plant
  • Plants, Genetically Modified
  • Plant Proteins
  • Plant Growth Regulators
 

Citation

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Silverstone, A. L., Jung, H. S., Dill, A., Kawaide, H., Kamiya, Y., & Sun, T. P. (2001). Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. The Plant Cell, 13(7), 1555–1566. https://doi.org/10.1105/tpc.010047
Silverstone, A. L., H. S. Jung, A. Dill, H. Kawaide, Y. Kamiya, and T. P. Sun. “Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis.The Plant Cell 13, no. 7 (July 2001): 1555–66. https://doi.org/10.1105/tpc.010047.
Silverstone AL, Jung HS, Dill A, Kawaide H, Kamiya Y, Sun TP. Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. The Plant cell. 2001 Jul;13(7):1555–66.
Silverstone, A. L., et al. “Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis.The Plant Cell, vol. 13, no. 7, July 2001, pp. 1555–66. Epmc, doi:10.1105/tpc.010047.
Silverstone AL, Jung HS, Dill A, Kawaide H, Kamiya Y, Sun TP. Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis. The Plant cell. 2001 Jul;13(7):1555–1566.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

July 2001

Volume

13

Issue

7

Start / End Page

1555 / 1566

Related Subject Headings

  • Transcription Factors
  • Suppression, Genetic
  • Signal Transduction
  • Sequence Alignment
  • Repressor Proteins
  • Recombinant Fusion Proteins
  • RNA, Plant
  • Plants, Genetically Modified
  • Plant Proteins
  • Plant Growth Regulators