Skip to main content
Journal cover image

Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.

Publication ,  Journal Article
Zhang, Y; Fan, W; Kinkema, M; Li, X; Dong, X
Published in: Proceedings of the National Academy of Sciences of the United States of America
May 1999

The Arabidopsis thaliana NPR1 has been shown to be a key regulator of gene expression during the onset of a plant disease-resistance response known as systemic acquired resistance. The npr1 mutant plants fail to respond to systemic acquired resistance-inducing signals such as salicylic acid (SA), or express SA-induced pathogenesis-related (PR) genes. Using NPR1 as bait in a yeast two-hybrid screen, we identified a subclass of transcription factors in the basic leucine zipper protein family (AHBP-1b and TGA6) and showed that they interact specifically in yeast and in vitro with NPR1. Point mutations that abolish the NPR1 function in A. thaliana also impair the interactions between NPR1 and the transcription factors in the yeast two-hybrid assay. Furthermore, a gel mobility shift assay showed that the purified transcription factor protein, AHBP-1b, binds specifically to an SA-responsive promoter element of the A. thaliana PR-1 gene. These data suggest that NPR1 may regulate PR-1 gene expression by interacting with a subclass of basic leucine zipper protein transcription factors.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

May 1999

Volume

96

Issue

11

Start / End Page

6523 / 6528

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Spodoptera
  • Solanum lycopersicum
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Salicylic Acid
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhang, Y., Fan, W., Kinkema, M., Li, X., & Dong, X. (1999). Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proceedings of the National Academy of Sciences of the United States of America, 96(11), 6523–6528. https://doi.org/10.1073/pnas.96.11.6523
Zhang, Y., W. Fan, M. Kinkema, X. Li, and X. Dong. “Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.Proceedings of the National Academy of Sciences of the United States of America 96, no. 11 (May 1999): 6523–28. https://doi.org/10.1073/pnas.96.11.6523.
Zhang Y, Fan W, Kinkema M, Li X, Dong X. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proceedings of the National Academy of Sciences of the United States of America. 1999 May;96(11):6523–8.
Zhang, Y., et al. “Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene.Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 11, May 1999, pp. 6523–28. Epmc, doi:10.1073/pnas.96.11.6523.
Zhang Y, Fan W, Kinkema M, Li X, Dong X. Interaction of NPR1 with basic leucine zipper protein transcription factors that bind sequences required for salicylic acid induction of the PR-1 gene. Proceedings of the National Academy of Sciences of the United States of America. 1999 May;96(11):6523–6528.
Journal cover image

Published In

Proceedings of the National Academy of Sciences of the United States of America

DOI

EISSN

1091-6490

ISSN

0027-8424

Publication Date

May 1999

Volume

96

Issue

11

Start / End Page

6523 / 6528

Related Subject Headings

  • Transfection
  • Transcription Factors
  • Spodoptera
  • Solanum lycopersicum
  • Sequence Homology, Amino Acid
  • Sequence Alignment
  • Salicylic Acid
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Recombinant Proteins