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Dissecting the architecture of a quantitative trait locus in yeast.

Publication ,  Journal Article
Steinmetz, LM; Sinha, H; Richards, DR; Spiegelman, JI; Oefner, PJ; McCusker, JH; Davis, RW
Published in: Nature
March 21, 2002

Most phenotypic diversity in natural populations is characterized by differences in degree rather than in kind. Identification of the actual genes underlying these quantitative traits has proved difficult. As a result, little is known about their genetic architecture. The failures are thought to be due to the different contributions of many underlying genes to the phenotype and the ability of different combinations of genes and environmental factors to produce similar phenotypes. This study combined genome-wide mapping and a new genetic technique named reciprocal-hemizygosity analysis to achieve the complete dissection of a quantitative trait locus (QTL) in Saccharomyces cerevisiae. A QTL architecture was uncovered that was more complex than expected. Functional linkages both in cis and in trans were found between three tightly linked quantitative trait genes that are neither necessary nor sufficient in isolation. This arrangement of alleles explains heterosis (hybrid vigour), the increased fitness of the heterozygote compared with homozygotes. It also demonstrates a deficiency in current approaches to QTL dissection with implications extending to traits in other organisms, including human genetic diseases.

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Published In

Nature

DOI

ISSN

0028-0836

Publication Date

March 21, 2002

Volume

416

Issue

6878

Start / End Page

326 / 330

Location

England

Related Subject Headings

  • Temperature
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Quantitative Trait, Heritable
  • Phenotype
  • Molecular Sequence Data
  • Hybridization, Genetic
  • Genotype
  • Genetic Techniques
  • Genetic Linkage
 

Citation

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Steinmetz, L. M., Sinha, H., Richards, D. R., Spiegelman, J. I., Oefner, P. J., McCusker, J. H., & Davis, R. W. (2002). Dissecting the architecture of a quantitative trait locus in yeast. Nature, 416(6878), 326–330. https://doi.org/10.1038/416326a
Steinmetz, Lars M., Himanshu Sinha, Dan R. Richards, Jamie I. Spiegelman, Peter J. Oefner, John H. McCusker, and Ronald W. Davis. “Dissecting the architecture of a quantitative trait locus in yeast.Nature 416, no. 6878 (March 21, 2002): 326–30. https://doi.org/10.1038/416326a.
Steinmetz LM, Sinha H, Richards DR, Spiegelman JI, Oefner PJ, McCusker JH, et al. Dissecting the architecture of a quantitative trait locus in yeast. Nature. 2002 Mar 21;416(6878):326–30.
Steinmetz, Lars M., et al. “Dissecting the architecture of a quantitative trait locus in yeast.Nature, vol. 416, no. 6878, Mar. 2002, pp. 326–30. Pubmed, doi:10.1038/416326a.
Steinmetz LM, Sinha H, Richards DR, Spiegelman JI, Oefner PJ, McCusker JH, Davis RW. Dissecting the architecture of a quantitative trait locus in yeast. Nature. 2002 Mar 21;416(6878):326–330.
Journal cover image

Published In

Nature

DOI

ISSN

0028-0836

Publication Date

March 21, 2002

Volume

416

Issue

6878

Start / End Page

326 / 330

Location

England

Related Subject Headings

  • Temperature
  • Spores, Fungal
  • Saccharomyces cerevisiae
  • Quantitative Trait, Heritable
  • Phenotype
  • Molecular Sequence Data
  • Hybridization, Genetic
  • Genotype
  • Genetic Techniques
  • Genetic Linkage