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The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.

Publication ,  Journal Article
Balzi, E; Chen, WN; Capieaux, E; McCusker, JH; Haber, JE; Goffeau, A
Published in: Gene
November 30, 1989

In Saccharomyces cerevisiae, the SCL-1 mutation is a dominant suppressor of the cycloheximide-resistant, temperature-sensitive (ts) lethal mutation, crl3 [McCusker and Haber, Genetics 119 (1988a) 303-315]. The wild-type scl1+ gene was isolated by screening subclones of the 35-kb region between TRP5 and LEU1 for restoration of the ts phenotype in an SCL1-1 crl3-2 strain. The scl1+ mRNA is about 900 nt long and encodes an open reading frame of 810 bp. The polypeptide deduced from scl1+ possesses a putative secretory signal peptide. The 5'-noncoding region may be under multiple controls, since it contains significant homology to the consensus sequences for the DNA-binding proteins, GCN4, GFI and, possibly, TUF. Gene disruption of scl1+ demonstrates that it is an essential gene.

Duke Scholars

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

November 30, 1989

Volume

83

Issue

2

Start / End Page

271 / 279

Location

Netherlands

Related Subject Headings

  • Transformation, Genetic
  • Suppression, Genetic
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • RNA, Fungal
  • Plasmids
  • Mutation
  • Molecular Sequence Data
  • Genes, Lethal
  • Genes, Fungal
 

Citation

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Balzi, E., Chen, W. N., Capieaux, E., McCusker, J. H., Haber, J. E., & Goffeau, A. (1989). The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Gene, 83(2), 271–279. https://doi.org/10.1016/0378-1119(89)90113-3
Balzi, E., W. N. Chen, E. Capieaux, J. H. McCusker, J. E. Haber, and A. Goffeau. “The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.Gene 83, no. 2 (November 30, 1989): 271–79. https://doi.org/10.1016/0378-1119(89)90113-3.
Balzi E, Chen WN, Capieaux E, McCusker JH, Haber JE, Goffeau A. The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Gene. 1989 Nov 30;83(2):271–9.
Balzi, E., et al. “The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth.Gene, vol. 83, no. 2, Nov. 1989, pp. 271–79. Pubmed, doi:10.1016/0378-1119(89)90113-3.
Balzi E, Chen WN, Capieaux E, McCusker JH, Haber JE, Goffeau A. The suppressor gene scl1+ of Saccharomyces cerevisiae is essential for growth. Gene. 1989 Nov 30;83(2):271–279.
Journal cover image

Published In

Gene

DOI

ISSN

0378-1119

Publication Date

November 30, 1989

Volume

83

Issue

2

Start / End Page

271 / 279

Location

Netherlands

Related Subject Headings

  • Transformation, Genetic
  • Suppression, Genetic
  • Saccharomyces cerevisiae
  • Restriction Mapping
  • RNA, Fungal
  • Plasmids
  • Mutation
  • Molecular Sequence Data
  • Genes, Lethal
  • Genes, Fungal