Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.
Publication
, Journal Article
Winzeler, EA; Shoemaker, DD; Astromoff, A; Liang, H; Anderson, K; Andre, B; Bangham, R; Benito, R; Boeke, JD; Bussey, H; Chu, AM; Connelly, C ...
Published in: Science
August 6, 1999
The functions of many open reading frames (ORFs) identified in genome-sequencing projects are unknown. New, whole-genome approaches are required to systematically determine their function. A total of 6925 Saccharomyces cerevisiae strains were constructed, by a high-throughput strategy, each with a precise deletion of one of 2026 ORFs (more than one-third of the ORFs in the genome). Of the deleted ORFs, 17 percent were essential for viability in rich medium. The phenotypes of more than 500 deletion strains were assayed in parallel. Of the deletion strains, 40 percent showed quantitative growth defects in either rich or minimal medium.
Duke Scholars
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Published In
Science
DOI
ISSN
0036-8075
Publication Date
August 6, 1999
Volume
285
Issue
5429
Start / End Page
901 / 906
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Recombination, Genetic
- Polymerase Chain Reaction
- Phenotype
- Open Reading Frames
- Genome, Fungal
- Genes, Fungal
- Genes, Essential
- General Science & Technology
- Gene Targeting
Citation
APA
Chicago
ICMJE
MLA
NLM
Winzeler, E. A., Shoemaker, D. D., Astromoff, A., Liang, H., Anderson, K., Andre, B., … Davis, R. W. (1999). Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science, 285(5429), 901–906. https://doi.org/10.1126/science.285.5429.901
Winzeler, E. A., D. D. Shoemaker, A. Astromoff, H. Liang, K. Anderson, B. Andre, R. Bangham, et al. “Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.” Science 285, no. 5429 (August 6, 1999): 901–6. https://doi.org/10.1126/science.285.5429.901.
Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B, et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 1999 Aug 6;285(5429):901–6.
Winzeler, E. A., et al. “Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis.” Science, vol. 285, no. 5429, Aug. 1999, pp. 901–06. Pubmed, doi:10.1126/science.285.5429.901.
Winzeler EA, Shoemaker DD, Astromoff A, Liang H, Anderson K, Andre B, Bangham R, Benito R, Boeke JD, Bussey H, Chu AM, Connelly C, Davis K, Dietrich F, Dow SW, El Bakkoury M, Foury F, Friend SH, Gentalen E, Giaever G, Hegemann JH, Jones T, Laub M, Liao H, Liebundguth N, Lockhart DJ, Lucau-Danila A, Lussier M, M’Rabet N, Menard P, Mittmann M, Pai C, Rebischung C, Revuelta JL, Riles L, Roberts CJ, Ross-MacDonald P, Scherens B, Snyder M, Sookhai-Mahadeo S, Storms RK, Véronneau S, Voet M, Volckaert G, Ward TR, Wysocki R, Yen GS, Yu K, Zimmermann K, Philippsen P, Johnston M, Davis RW. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science. 1999 Aug 6;285(5429):901–906.
Published In
Science
DOI
ISSN
0036-8075
Publication Date
August 6, 1999
Volume
285
Issue
5429
Start / End Page
901 / 906
Location
United States
Related Subject Headings
- Saccharomyces cerevisiae
- Recombination, Genetic
- Polymerase Chain Reaction
- Phenotype
- Open Reading Frames
- Genome, Fungal
- Genes, Fungal
- Genes, Essential
- General Science & Technology
- Gene Targeting