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Nuclear localization of NPR1 is required for activation of PR gene expression.

Publication ,  Journal Article
Kinkema, M; Fan, W; Dong, X
Published in: The Plant cell
December 2000

Systemic acquired resistance (SAR) is a broad-spectrum resistance in plants that involves the upregulation of a battery of pathogenesis-related (PR) genes. NPR1 is a key regulator in the signal transduction pathway that leads to SAR. Mutations in NPR1 result in a failure to induce PR genes in systemic tissues and a heightened susceptibility to pathogen infection, whereas overexpression of the NPR1 protein leads to increased induction of the PR genes and enhanced disease resistance. We analyzed the subcellular localization of NPR1 to gain insight into the mechanism by which this protein regulates SAR. An NPR1-green fluorescent protein fusion protein, which functions the same as the endogenous NPR1 protein, was shown to accumulate in the nucleus in response to activators of SAR. To control the nuclear transport of NPR1, we made a fusion of NPR1 with the glucocorticoid receptor hormone binding domain. Using this steroid-inducible system, we clearly demonstrate that nuclear localization of NPR1 is essential for its activity in inducing PR genes.

Duke Scholars

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

December 2000

Volume

12

Issue

12

Start / End Page

2339 / 2350

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Protein Kinases
  • Plant Proteins
  • Plant Biology & Botany
  • Nuclear Localization Signals
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Gene Expression Regulation, Plant
  • Fungal Proteins
 

Citation

APA
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ICMJE
MLA
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Kinkema, M., Fan, W., & Dong, X. (2000). Nuclear localization of NPR1 is required for activation of PR gene expression. The Plant Cell, 12(12), 2339–2350. https://doi.org/10.1105/tpc.12.12.2339
Kinkema, M., W. Fan, and X. Dong. “Nuclear localization of NPR1 is required for activation of PR gene expression.The Plant Cell 12, no. 12 (December 2000): 2339–50. https://doi.org/10.1105/tpc.12.12.2339.
Kinkema M, Fan W, Dong X. Nuclear localization of NPR1 is required for activation of PR gene expression. The Plant cell. 2000 Dec;12(12):2339–50.
Kinkema, M., et al. “Nuclear localization of NPR1 is required for activation of PR gene expression.The Plant Cell, vol. 12, no. 12, Dec. 2000, pp. 2339–50. Epmc, doi:10.1105/tpc.12.12.2339.
Kinkema M, Fan W, Dong X. Nuclear localization of NPR1 is required for activation of PR gene expression. The Plant cell. 2000 Dec;12(12):2339–2350.

Published In

The Plant cell

DOI

EISSN

1532-298X

ISSN

1040-4651

Publication Date

December 2000

Volume

12

Issue

12

Start / End Page

2339 / 2350

Related Subject Headings

  • Saccharomyces cerevisiae Proteins
  • Recombinant Fusion Proteins
  • Protein Kinases
  • Plant Proteins
  • Plant Biology & Botany
  • Nuclear Localization Signals
  • Luminescent Proteins
  • Green Fluorescent Proteins
  • Gene Expression Regulation, Plant
  • Fungal Proteins