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Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin.

Publication ,  Journal Article
Schwartz, CL; Minniti, CP; Harwood, P; Na, S; Banquerigo, ML; Strauss, LC; Kurtzberg, J; Smith, SD; Civin, CI
Published in: J Clin Oncol
December 1987

2'Deoxycoformycin (dCF) specifically inhibits adenosine deaminase (ADA) and causes selective cytotoxicity of normal and malignant T cells. In clinical trials, dCF caused rapid lysis of malignant T lymphoblasts. Although dCF has been associated with dose-limiting nonhematopoietic toxicities, myelosuppression has not been observed. Since dCF is relatively nontoxic to hematopoietic stem cells, we tested dCF for utility in the ex vivo purging of malignant T lymphoblasts from remission leukemic bone marrow for autologous bone marrow transplantation. We found that T lymphoblast cell lines were sensitive to dCF (plus deoxyadenosine [dAdo]) under conditions that did not ablate human hematopoietic colony-forming cells. Moreover, combined pharmacologic (dCF plus dAdo) and immunologic (anti-T cell monoclonal antibodies [McAb] plus complement) purging resulted in additive reduction in clonogenic T lymphoblasts. These results provide the basis for a clinical trial of bone marrow transplantation using combined pharmacologic/immunologic purging of T lymphoblasts from patients' harvested autologous marrow.

Duke Scholars

Published In

J Clin Oncol

DOI

ISSN

0732-183X

Publication Date

December 1987

Volume

5

Issue

12

Start / End Page

1900 / 1911

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Ribonucleosides
  • Pentostatin
  • Oncology & Carcinogenesis
  • Lymphoma
  • Lymphocyte Depletion
  • Humans
  • Deoxyadenosines
  • Complement System Proteins
  • Colony-Forming Units Assay
 

Citation

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Schwartz, C. L., Minniti, C. P., Harwood, P., Na, S., Banquerigo, M. L., Strauss, L. C., … Civin, C. I. (1987). Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin. J Clin Oncol, 5(12), 1900–1911. https://doi.org/10.1200/JCO.1987.5.12.1900
Schwartz, C. L., C. P. Minniti, P. Harwood, S. Na, M. L. Banquerigo, L. C. Strauss, J. Kurtzberg, S. D. Smith, and C. I. Civin. “Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin.J Clin Oncol 5, no. 12 (December 1987): 1900–1911. https://doi.org/10.1200/JCO.1987.5.12.1900.
Schwartz CL, Minniti CP, Harwood P, Na S, Banquerigo ML, Strauss LC, et al. Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin. J Clin Oncol. 1987 Dec;5(12):1900–11.
Schwartz, C. L., et al. “Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin.J Clin Oncol, vol. 5, no. 12, Dec. 1987, pp. 1900–11. Pubmed, doi:10.1200/JCO.1987.5.12.1900.
Schwartz CL, Minniti CP, Harwood P, Na S, Banquerigo ML, Strauss LC, Kurtzberg J, Smith SD, Civin CI. Elimination of clonogenic malignant human T cells using monoclonal antibodies in combination with 2'-deoxycoformycin. J Clin Oncol. 1987 Dec;5(12):1900–1911.

Published In

J Clin Oncol

DOI

ISSN

0732-183X

Publication Date

December 1987

Volume

5

Issue

12

Start / End Page

1900 / 1911

Location

United States

Related Subject Headings

  • T-Lymphocytes
  • Ribonucleosides
  • Pentostatin
  • Oncology & Carcinogenesis
  • Lymphoma
  • Lymphocyte Depletion
  • Humans
  • Deoxyadenosines
  • Complement System Proteins
  • Colony-Forming Units Assay