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Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry.

Publication ,  Journal Article
Fitzgerald, MC; Smith, LM
Published in: Annual review of biophysics and biomolecular structure
January 1995

In the past several years, significant progress has been made in the application of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry to the analysis of large biopolymers, including nucleic acids. By isolating analyte molecules in an appropriate matrix and irradiating the sample with a high-intensity, pulsed laser beam, MALDI can generate intact, gas-phase ions of these analytes. Primarily used with time-of-flight mass spectrometers, this relatively new, soft ionization technique has allowed for the routine analysis of oligonucleotides up to 60 or so nucleotides in length. Recent results have also shown that base specific, matrix-dependent fragmentation is an important factor in the MALDI analysis of oligonucleotides. Further extension of the technique to longer oligonucleotides will rely on both the continued search for new matrix materials and an increased understanding of the desorption and ionization process in MALDI.

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Published In

Annual review of biophysics and biomolecular structure

DOI

EISSN

1545-4266

ISSN

1056-8700

Publication Date

January 1995

Volume

24

Start / End Page

117 / 140

Related Subject Headings

  • Sequence Analysis, DNA
  • Oligodeoxyribonucleotides
  • Nucleic Acids
  • Molecular Sequence Data
  • Mass Spectrometry
  • Lasers
  • Ions
  • Biophysics
  • Base Sequence
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Fitzgerald, M. C., & Smith, L. M. (1995). Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry. Annual Review of Biophysics and Biomolecular Structure, 24, 117–140. https://doi.org/10.1146/annurev.bb.24.060195.001001
Fitzgerald, M. C., and L. M. Smith. “Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry.Annual Review of Biophysics and Biomolecular Structure 24 (January 1995): 117–40. https://doi.org/10.1146/annurev.bb.24.060195.001001.
Fitzgerald MC, Smith LM. Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry. Annual review of biophysics and biomolecular structure. 1995 Jan;24:117–40.
Fitzgerald, M. C., and L. M. Smith. “Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry.Annual Review of Biophysics and Biomolecular Structure, vol. 24, Jan. 1995, pp. 117–40. Epmc, doi:10.1146/annurev.bb.24.060195.001001.
Fitzgerald MC, Smith LM. Mass spectrometry of nucleic acids: the promise of matrix-assisted laser desorption-ionization (MALDI) mass spectrometry. Annual review of biophysics and biomolecular structure. 1995 Jan;24:117–140.

Published In

Annual review of biophysics and biomolecular structure

DOI

EISSN

1545-4266

ISSN

1056-8700

Publication Date

January 1995

Volume

24

Start / End Page

117 / 140

Related Subject Headings

  • Sequence Analysis, DNA
  • Oligodeoxyribonucleotides
  • Nucleic Acids
  • Molecular Sequence Data
  • Mass Spectrometry
  • Lasers
  • Ions
  • Biophysics
  • Base Sequence
  • 3404 Medicinal and biomolecular chemistry