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Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases.

Publication ,  Journal Article
Weinman, EJ; Dubinsky, WP; Fisher, K; Steplock, D; Dinh, Q; Chang, L; Shenolikar, S
Published in: J Membr Biol
August 1988

Studies were performed to determine the effect of protein phosphorylation mediated by calcium-calmodulin-dependent multifunctional protein kinase II and calcium-phospholipid-dependent protein kinase on Na+/H+ exchange activity. Proteins from the apical membrane of the proximal tubule of the rabbit kidney were solubilized in octyl glucoside and incubated in phosphorylating solutions containing the protein kinase. 22Na+ uptake was determined subsequently after reconstitution of the proteins into proteoliposomes. Calcium-calmodulin-dependent multifunction protein kinase II inhibited the amiloride-sensitive component of proton gradient-stimulated Na+ uptake in a dose-dependent manner. The inhibitory effect of this kinase had an absolute requirement for calmodulin, Ca2+, and ATP. Calcium-phospholipid-dependent protein kinase stimulated the amiloride-sensitive component of proton gradient-stimulated Na+ uptake in a dose-dependent manner. The stimulating effect of this kinase had an absolute requirement for ATP, Ca2+, and an active phorbol ester. These experiments indicate that Na+/H+ exchange activity of proteoliposomes reconstituted with proteins from renal brush-border membranes are inhibited by protein phosphorylation mediated by calcium-calmodulin-dependent multifunctional protein kinase II and stimulated by that mediated by calcium-calmodulin-dependent protein kinase.

Duke Scholars

Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

August 1988

Volume

103

Issue

3

Start / End Page

237 / 244

Location

United States

Related Subject Headings

  • Sodium-Hydrogen Exchangers
  • Rabbits
  • Protein Kinases
  • Protein Kinase C
  • Physiology
  • Phosphorylation
  • Microvilli
  • Kidney Tubules, Proximal
  • Hydrogen-Ion Concentration
  • Electrophoresis, Polyacrylamide Gel
 

Citation

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ICMJE
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Weinman, E. J., Dubinsky, W. P., Fisher, K., Steplock, D., Dinh, Q., Chang, L., & Shenolikar, S. (1988). Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases. J Membr Biol, 103(3), 237–244. https://doi.org/10.1007/BF01993983
Weinman, E. J., W. P. Dubinsky, K. Fisher, D. Steplock, Q. Dinh, L. Chang, and S. Shenolikar. “Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases.J Membr Biol 103, no. 3 (August 1988): 237–44. https://doi.org/10.1007/BF01993983.
Weinman EJ, Dubinsky WP, Fisher K, Steplock D, Dinh Q, Chang L, et al. Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases. J Membr Biol. 1988 Aug;103(3):237–44.
Weinman, E. J., et al. “Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases.J Membr Biol, vol. 103, no. 3, Aug. 1988, pp. 237–44. Pubmed, doi:10.1007/BF01993983.
Weinman EJ, Dubinsky WP, Fisher K, Steplock D, Dinh Q, Chang L, Shenolikar S. Regulation of reconstituted renal Na+/H+ exchanger by calcium-dependent protein kinases. J Membr Biol. 1988 Aug;103(3):237–244.
Journal cover image

Published In

J Membr Biol

DOI

ISSN

0022-2631

Publication Date

August 1988

Volume

103

Issue

3

Start / End Page

237 / 244

Location

United States

Related Subject Headings

  • Sodium-Hydrogen Exchangers
  • Rabbits
  • Protein Kinases
  • Protein Kinase C
  • Physiology
  • Phosphorylation
  • Microvilli
  • Kidney Tubules, Proximal
  • Hydrogen-Ion Concentration
  • Electrophoresis, Polyacrylamide Gel