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Chromosomal organization of rRNA operons in Bacillus subtilis.

Publication ,  Journal Article
Jarvis, ED; Widom, RL; LaFauci, G; Setoguchi, Y; Richter, IR; Rudner, R
Published in: Genetics
November 1988

Integrative mapping with vectors containing ribosomal DNA sequences were used to complete the mapping of the 10 rRNA gene sets in the endospore forming bacterium Bacillus subtilis. Southern hybridizations allowed the assignment of nine operons to distinct BclI restriction fragments and their genetic locus identified by transductional crosses. Nine of the ten rRNA gene sets are located between 0 and 70 degrees on the genomic map. In the region surrounding cysA14, two sets of closely spaced tandem clusters are present. The first (rrnJ and rrnW) is located between purA16 and cysA14 closely linked to the latter; the second (rrnI, rrnH and rrnG) previously mapped within this area is located between attSPO2 and glpT6. The operons at or near the origin of replication (rrnO,rrnA and rrnJ,rrnW) represent "hot spots" of plasmid insertion.

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Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 1988

Volume

120

Issue

3

Start / End Page

625 / 635

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Transduction, Genetic
  • Restriction Mapping
  • RNA, Ribosomal
  • RNA, Bacterial
  • Plasmids
  • Operon
  • Multigene Family
  • Genetic Linkage
  • Genes, Bacterial
 

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Jarvis, E. D., Widom, R. L., LaFauci, G., Setoguchi, Y., Richter, I. R., & Rudner, R. (1988). Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics, 120(3), 625–635. https://doi.org/10.1093/genetics/120.3.625
Jarvis, E. D., R. L. Widom, G. LaFauci, Y. Setoguchi, I. R. Richter, and R. Rudner. “Chromosomal organization of rRNA operons in Bacillus subtilis.Genetics 120, no. 3 (November 1988): 625–35. https://doi.org/10.1093/genetics/120.3.625.
Jarvis ED, Widom RL, LaFauci G, Setoguchi Y, Richter IR, Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics. 1988 Nov;120(3):625–35.
Jarvis, E. D., et al. “Chromosomal organization of rRNA operons in Bacillus subtilis.Genetics, vol. 120, no. 3, Nov. 1988, pp. 625–35. Pubmed, doi:10.1093/genetics/120.3.625.
Jarvis ED, Widom RL, LaFauci G, Setoguchi Y, Richter IR, Rudner R. Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics. 1988 Nov;120(3):625–635.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 1988

Volume

120

Issue

3

Start / End Page

625 / 635

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Transduction, Genetic
  • Restriction Mapping
  • RNA, Ribosomal
  • RNA, Bacterial
  • Plasmids
  • Operon
  • Multigene Family
  • Genetic Linkage
  • Genes, Bacterial