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Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.

Publication ,  Journal Article
Rudner, R; Chevrestt, A; Buchholz, SR; Studamire, B; White, AM; Jarvis, ED
Published in: J Bacteriol
January 1993

Sequence analysis of cloned rescued DNA fragments from a Bacillus subtilis strain with an inserted recombinant plasmid in ribosomal operon rrnE revealed the presence of two tRNA genes for Met and Asp at the 3' end of the operon. Probing chromosomal DNA from a strain carrying a plasmid inserted in rrnD with a fragment containing the genetically unassigned cluster of 16 tRNA genes revealed that the cluster is located immediately following the rrnD operon. Our findings show that all 10 rrn operons in B. subtilis are associated with tRNA gene clusters.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1993

Volume

175

Issue

2

Start / End Page

503 / 509

Location

United States

Related Subject Headings

  • Restriction Mapping
  • RNA, Transfer
  • RNA, Ribosomal
  • Polymerase Chain Reaction
  • Operon
  • Oligodeoxyribonucleotides
  • Multigene Family
  • Molecular Sequence Data
  • Microbiology
  • Genes, Bacterial
 

Citation

APA
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ICMJE
MLA
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Rudner, R., Chevrestt, A., Buchholz, S. R., Studamire, B., White, A. M., & Jarvis, E. D. (1993). Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis. J Bacteriol, 175(2), 503–509. https://doi.org/10.1128/jb.175.2.503-509.1993
Rudner, R., A. Chevrestt, S. R. Buchholz, B. Studamire, A. M. White, and E. D. Jarvis. “Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.J Bacteriol 175, no. 2 (January 1993): 503–9. https://doi.org/10.1128/jb.175.2.503-509.1993.
Rudner R, Chevrestt A, Buchholz SR, Studamire B, White AM, Jarvis ED. Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis. J Bacteriol. 1993 Jan;175(2):503–9.
Rudner, R., et al. “Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis.J Bacteriol, vol. 175, no. 2, Jan. 1993, pp. 503–09. Pubmed, doi:10.1128/jb.175.2.503-509.1993.
Rudner R, Chevrestt A, Buchholz SR, Studamire B, White AM, Jarvis ED. Two tRNA gene clusters associated with rRNA operons rrnD and rrnE in Bacillus subtilis. J Bacteriol. 1993 Jan;175(2):503–509.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1993

Volume

175

Issue

2

Start / End Page

503 / 509

Location

United States

Related Subject Headings

  • Restriction Mapping
  • RNA, Transfer
  • RNA, Ribosomal
  • Polymerase Chain Reaction
  • Operon
  • Oligodeoxyribonucleotides
  • Multigene Family
  • Molecular Sequence Data
  • Microbiology
  • Genes, Bacterial