Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase.
Publication
, Journal Article
Wang, JX; Shaw, BR
Published in: Nucleosides, nucleotides & nucleic acids
January 2005
In order to introduce pyrimidine C5-propynyl modification into boranophosphate oligodeoxyribonucleotides (BP- ODNs), 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano) triphosphate (d5PUTPalphaB) was synthesized. The two diastereomers were separated by reverse-phase HPLC. Kinetic studies showed that the Rp isomer was a slightly better substrate for MMLV reverse transcriptase than thymidine triphosphate or Rp-thymidine 5'-(alpha-P-borano)triphosphate. Using the Rp isomers of d5PUTPalphaB and the other three 5'-(alpha-P-borano) triphosphates, a DNA primer could be extended to the full length of the template.
Duke Scholars
Published In
Nucleosides, nucleotides & nucleic acids
DOI
EISSN
1532-2335
ISSN
1525-7770
Publication Date
January 2005
Volume
24
Issue
5-7
Start / End Page
947 / 950
Related Subject Headings
- Uridine Triphosphate
- Uridine
- Thymine Nucleotides
- Thymidine
- RNA-Directed DNA Polymerase
- Pyrimidines
- Protein Isoforms
- Polyphosphates
- Organic Chemistry
- Oligonucleotides
Citation
APA
Chicago
ICMJE
MLA
NLM
Wang, J. X., & Shaw, B. R. (2005). Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase. Nucleosides, Nucleotides & Nucleic Acids, 24(5–7), 947–950. https://doi.org/10.1081/ncn-200059307
Wang, Joy Xin, and Barbara Ramsay Shaw. “Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase.” Nucleosides, Nucleotides & Nucleic Acids 24, no. 5–7 (January 2005): 947–50. https://doi.org/10.1081/ncn-200059307.
Wang JX, Shaw BR. Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase. Nucleosides, nucleotides & nucleic acids. 2005 Jan;24(5–7):947–50.
Wang, Joy Xin, and Barbara Ramsay Shaw. “Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase.” Nucleosides, Nucleotides & Nucleic Acids, vol. 24, no. 5–7, Jan. 2005, pp. 947–50. Epmc, doi:10.1081/ncn-200059307.
Wang JX, Shaw BR. Synthesis of 5-(1-propynyl)-2'-deoxyuridine 5'-(alpha-P-borano)triphosphate and kinetic characterization as a substrate for mmlv reverse transcriptase. Nucleosides, nucleotides & nucleic acids. 2005 Jan;24(5–7):947–950.
Published In
Nucleosides, nucleotides & nucleic acids
DOI
EISSN
1532-2335
ISSN
1525-7770
Publication Date
January 2005
Volume
24
Issue
5-7
Start / End Page
947 / 950
Related Subject Headings
- Uridine Triphosphate
- Uridine
- Thymine Nucleotides
- Thymidine
- RNA-Directed DNA Polymerase
- Pyrimidines
- Protein Isoforms
- Polyphosphates
- Organic Chemistry
- Oligonucleotides