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DNA typing for HLA-A and HLA-B identifies disparities between patients and unrelated donors matched by HLA-A and HLA-B serology and HLA-DRB1.

Publication ,  Journal Article
Prasad, VK; Kernan, NA; Heller, G; O'Reilly, RJ; Yang, SY
Published in: Blood
January 1, 1999

High incidences of graft failure and graft-versus-host disease in the recipients of bone marrow transplantations (BMT) from unrelated donors (URD) may reflect the existence of allelic disparities between the patient and the URD despite apparent HLA identity at HLA-A, HLA-B, and HLA-DRB1 loci. To identify the extent and pattern of allelic disparities at HLA-A and HLA-B loci, 128 patients and 484 potential URD were evaluated by DNA typing. DNA typing for HLA-A, HLA-B, and HLA-DRB1 was performed at Memorial Sloan Kettering Cancer Center. HLA-A and HLA-B serotyping on URD was provided by the registries. By original typing (serology for HLA-A and HLA-B; DNA typing for DRB1) 187, 164, and 133 URD were 6/6, 5/6, and 4/6 matches, respectively. Following DNA typing, however, only 52.9% of the originally 6/6 matched URD remained 6/6, while 38.5%, 7.5%, and 1.1% were found to be 5/6, 4/6, and 3/6 matches. The level of disparity was higher in the originally 5/6 (P <.01) and 4/6 (P <.01) matched URD. A higher level of disparity was seen for HLA-B as compared to HLA-A. In addition, a serotype related variation was also noticed. For example, 24.1% of HLA-A2 and 60.1% of HLA-B35 seromatched URD were genotypically disparate, but no disparities were seen for HLA-A1 and HLA-B8. A higher percentage of HLA-A (67. 4%) compared with HLA-B (35.4%) serologic homozygous URD remained genotypically homozygous (P =.01). The level of allelic disparity was lower (P <.01 for 6/6; P =.02 for 5/6) if the patient had one of the 15 most common haplotypes (A1B8DR3, A2B7DR15, A3B7DR15, etc) in comparison to the rest of the group. Outcome studies will answer the question whether these disparities are associated with a higher rate of immunological complications seen with URD-BMT.

Duke Scholars

Published In

Blood

ISSN

0006-4971

Publication Date

January 1, 1999

Volume

93

Issue

1

Start / End Page

399 / 409

Location

United States

Related Subject Headings

  • Tissue Donors
  • Racial Groups
  • Polymerase Chain Reaction
  • Immunology
  • Humans
  • Histocompatibility Testing
  • Haplotypes
  • HLA-DRB1 Chains
  • HLA-DR Antigens
  • HLA-B Antigens
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Prasad, V. K., Kernan, N. A., Heller, G., O’Reilly, R. J., & Yang, S. Y. (1999). DNA typing for HLA-A and HLA-B identifies disparities between patients and unrelated donors matched by HLA-A and HLA-B serology and HLA-DRB1. Blood, 93(1), 399–409.
Prasad, V. K., N. A. Kernan, G. Heller, R. J. O’Reilly, and S. Y. Yang. “DNA typing for HLA-A and HLA-B identifies disparities between patients and unrelated donors matched by HLA-A and HLA-B serology and HLA-DRB1.Blood 93, no. 1 (January 1, 1999): 399–409.

Published In

Blood

ISSN

0006-4971

Publication Date

January 1, 1999

Volume

93

Issue

1

Start / End Page

399 / 409

Location

United States

Related Subject Headings

  • Tissue Donors
  • Racial Groups
  • Polymerase Chain Reaction
  • Immunology
  • Humans
  • Histocompatibility Testing
  • Haplotypes
  • HLA-DRB1 Chains
  • HLA-DR Antigens
  • HLA-B Antigens