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Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms.

Publication ,  Journal Article
Bonni, A; Brunet, A; West, AE; Datta, SR; Takasu, MA; Greenberg, ME
Published in: Science
November 12, 1999

A mechanism by which the Ras-mitogen-activated protein kinase (MAPK) signaling pathway mediates growth factor-dependent cell survival was characterized. The MAPK-activated kinases, the Rsks, catalyzed the phosphorylation of the pro-apoptotic protein BAD at serine 112 both in vitro and in vivo. The Rsk-induced phosphorylation of BAD at serine 112 suppressed BAD-mediated apoptosis in neurons. Rsks also are known to phosphorylate the transcription factor CREB (cAMP response element-binding protein) at serine 133. Activated CREB promoted cell survival, and inhibition of CREB phosphorylation at serine 133 triggered apoptosis. These findings suggest that the MAPK signaling pathway promotes cell survival by a dual mechanism comprising the posttranslational modification and inactivation of a component of the cell death machinery and the increased transcription of pro-survival genes.

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Published In

Science

DOI

ISSN

0036-8075

Publication Date

November 12, 1999

Volume

286

Issue

5443

Start / End Page

1358 / 1362

Location

United States

Related Subject Headings

  • ras Proteins
  • bcl-Associated Death Protein
  • Transfection
  • Transcription, Genetic
  • Ribosomal Protein S6 Kinases
  • Recombinant Fusion Proteins
  • Rats, Long-Evans
  • Rats
  • Protein Serine-Threonine Kinases
  • Phosphoserine
 

Citation

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Bonni, A., Brunet, A., West, A. E., Datta, S. R., Takasu, M. A., & Greenberg, M. E. (1999). Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science, 286(5443), 1358–1362. https://doi.org/10.1126/science.286.5443.1358
Bonni, A., A. Brunet, A. E. West, S. R. Datta, M. A. Takasu, and M. E. Greenberg. “Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms.Science 286, no. 5443 (November 12, 1999): 1358–62. https://doi.org/10.1126/science.286.5443.1358.
Bonni A, Brunet A, West AE, Datta SR, Takasu MA, Greenberg ME. Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science. 1999 Nov 12;286(5443):1358–62.
Bonni, A., et al. “Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms.Science, vol. 286, no. 5443, Nov. 1999, pp. 1358–62. Pubmed, doi:10.1126/science.286.5443.1358.
Bonni A, Brunet A, West AE, Datta SR, Takasu MA, Greenberg ME. Cell survival promoted by the Ras-MAPK signaling pathway by transcription-dependent and -independent mechanisms. Science. 1999 Nov 12;286(5443):1358–1362.
Journal cover image

Published In

Science

DOI

ISSN

0036-8075

Publication Date

November 12, 1999

Volume

286

Issue

5443

Start / End Page

1358 / 1362

Location

United States

Related Subject Headings

  • ras Proteins
  • bcl-Associated Death Protein
  • Transfection
  • Transcription, Genetic
  • Ribosomal Protein S6 Kinases
  • Recombinant Fusion Proteins
  • Rats, Long-Evans
  • Rats
  • Protein Serine-Threonine Kinases
  • Phosphoserine