Skip to main content

Double-strand break repair in tandem repeats during bacteriophage T4 infection.

Publication ,  Journal Article
Tomso, DJ; Kreuzer, KN
Published in: Genetics
August 2000

Recombinational repair of double-strand breaks in tandemly repeated sequences often results in the loss of one or more copies of the repeat. The single-strand annealing (SSA) model for repair has been proposed to account for this nonconservative recombination. In this study we present a plasmid-based physical assay that measures SSA during bacteriophage T4 infection and apply this assay to the genetic analysis of break repair. SSA occurs readily in broken plasmid DNA and is independent of the strand exchange protein UvsX and its accessory factor UvsY. We use the unique features of T4 DNA metabolism to examine the link between SSA repair and DNA replication and demonstrate directly that the DNA polymerase and the major replicative helicase of the phage are not required for SSA repair. We also show that the Escherichia coli RecBCD enzyme can mediate the degradation of broken DNA during early, but not late, times of infection. Finally, we consider the status of broken ends during the course of the infection and propose a model for SSA during T4 infections.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 2000

Volume

155

Issue

4

Start / End Page

1493 / 1504

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Time Factors
  • Tandem Repeat Sequences
  • Recombination, Genetic
  • Plasmids
  • Models, Genetic
  • Electrophoresis, Gel, Two-Dimensional
  • Developmental Biology
  • DNA, Viral
  • DNA Replication
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tomso, D. J., & Kreuzer, K. N. (2000). Double-strand break repair in tandem repeats during bacteriophage T4 infection. Genetics, 155(4), 1493–1504. https://doi.org/10.1093/genetics/155.4.1493
Tomso, D. J., and K. N. Kreuzer. “Double-strand break repair in tandem repeats during bacteriophage T4 infection.Genetics 155, no. 4 (August 2000): 1493–1504. https://doi.org/10.1093/genetics/155.4.1493.
Tomso DJ, Kreuzer KN. Double-strand break repair in tandem repeats during bacteriophage T4 infection. Genetics. 2000 Aug;155(4):1493–504.
Tomso, D. J., and K. N. Kreuzer. “Double-strand break repair in tandem repeats during bacteriophage T4 infection.Genetics, vol. 155, no. 4, Aug. 2000, pp. 1493–504. Pubmed, doi:10.1093/genetics/155.4.1493.
Tomso DJ, Kreuzer KN. Double-strand break repair in tandem repeats during bacteriophage T4 infection. Genetics. 2000 Aug;155(4):1493–1504.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

August 2000

Volume

155

Issue

4

Start / End Page

1493 / 1504

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Time Factors
  • Tandem Repeat Sequences
  • Recombination, Genetic
  • Plasmids
  • Models, Genetic
  • Electrophoresis, Gel, Two-Dimensional
  • Developmental Biology
  • DNA, Viral
  • DNA Replication