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The tight linkage between DNA replication and double-strand break repair in bacteriophage T4.

Publication ,  Journal Article
George, JW; Stohr, BA; Tomso, DJ; Kreuzer, KN
Published in: Proc Natl Acad Sci U S A
July 17, 2001

Double-strand break (DSB) repair and DNA replication are tightly linked in the life cycle of bacteriophage T4. Indeed, the major mode of phage DNA replication depends on recombination proteins and can be stimulated by DSBs. DSB-stimulated DNA replication is dramatically demonstrated when T4 infects cells carrying two plasmids that share homology. A DSB on one plasmid triggered extensive replication of the second plasmid, providing a useful model for T4 recombination-dependent replication (RDR). This system also provides a view of DSB repair in T4-infected cells and revealed that the DSB repair products had been replicated in their entirety by the T4 replication machinery. We analyzed the detailed structure of these products, which do not fit the simple predictions of any of three models for DSB repair. We also present evidence that the T4 RDR system functions to restart stalled or inactivated replication forks. First, we review experiments involving antitumor drug-stabilized topoisomerase cleavage complexes. The results suggest that forks blocked at cleavage complexes are resolved by recombinational repair, likely involving RDR. Second, we show here that the presence of a T4 replication origin on one plasmid substantially stimulated recombination events between it and a homologous second plasmid that did not contain a T4 origin. Furthermore, replication of the second plasmid was increased when the first plasmid contained the T4 origin. Our interpretation is that origin-initiated forks become inactivated at some frequency during replication of the first plasmid and are then restarted via RDR on the second plasmid.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 17, 2001

Volume

98

Issue

15

Start / End Page

8290 / 8297

Location

United States

Related Subject Headings

  • Viral Proteins
  • Replication Origin
  • Plasmids
  • Models, Genetic
  • DNA, Viral
  • DNA Topoisomerases, Type I
  • DNA Replication
  • DNA Repair
  • DNA Damage
  • Bacteriophage T4
 

Citation

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MLA
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George, J. W., Stohr, B. A., Tomso, D. J., & Kreuzer, K. N. (2001). The tight linkage between DNA replication and double-strand break repair in bacteriophage T4. Proc Natl Acad Sci U S A, 98(15), 8290–8297. https://doi.org/10.1073/pnas.131007598
George, J. W., B. A. Stohr, D. J. Tomso, and K. N. Kreuzer. “The tight linkage between DNA replication and double-strand break repair in bacteriophage T4.Proc Natl Acad Sci U S A 98, no. 15 (July 17, 2001): 8290–97. https://doi.org/10.1073/pnas.131007598.
George JW, Stohr BA, Tomso DJ, Kreuzer KN. The tight linkage between DNA replication and double-strand break repair in bacteriophage T4. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8290–7.
George, J. W., et al. “The tight linkage between DNA replication and double-strand break repair in bacteriophage T4.Proc Natl Acad Sci U S A, vol. 98, no. 15, July 2001, pp. 8290–97. Pubmed, doi:10.1073/pnas.131007598.
George JW, Stohr BA, Tomso DJ, Kreuzer KN. The tight linkage between DNA replication and double-strand break repair in bacteriophage T4. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8290–8297.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

July 17, 2001

Volume

98

Issue

15

Start / End Page

8290 / 8297

Location

United States

Related Subject Headings

  • Viral Proteins
  • Replication Origin
  • Plasmids
  • Models, Genetic
  • DNA, Viral
  • DNA Topoisomerases, Type I
  • DNA Replication
  • DNA Repair
  • DNA Damage
  • Bacteriophage T4