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Coordination of DNA ends during double-strand-break repair in bacteriophage T4.

Publication ,  Journal Article
Stohr, BA; Kreuzer, KN
Published in: Genetics
November 2002

The extensive chromosome replication (ECR) model of double-strand-break repair (DSBR) proposes that each end of a double-strand break (DSB) is repaired independently by initiating extensive semiconservative DNA replication after strand invasion into homologous template DNA. In contrast, several other DSBR models propose that the two ends of a break are repaired in a coordinated manner using a single repair template with only limited DNA synthesis. We have developed plasmid and chromosomal recombinational repair assays to assess coordination of the broken ends during DSBR in bacteriophage T4. Results from the plasmid assay demonstrate that the two ends of a DSB can be repaired independently using homologous regions on two different plasmids and that extensive replication is triggered in the process. These findings are consistent with the ECR model of DSBR. However, results from the chromosomal assay imply that the two ends of a DSB utilize the same homologous repair template even when many potential templates are present, suggesting coordination of the broken ends during chromosomal repair. This result is consistent with several coordinated models of DSBR, including a modified version of the ECR model.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 2002

Volume

162

Issue

3

Start / End Page

1019 / 1030

Location

United States

Related Subject Headings

  • Developmental Biology
  • DNA Repair
  • DNA
  • Chromosomes
  • Biological Assay
  • Bacteriophage T4
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 0604 Genetics
 

Citation

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Stohr, B. A., & Kreuzer, K. N. (2002). Coordination of DNA ends during double-strand-break repair in bacteriophage T4. Genetics, 162(3), 1019–1030. https://doi.org/10.1093/genetics/162.3.1019
Stohr, Bradley A., and Kenneth N. Kreuzer. “Coordination of DNA ends during double-strand-break repair in bacteriophage T4.Genetics 162, no. 3 (November 2002): 1019–30. https://doi.org/10.1093/genetics/162.3.1019.
Stohr BA, Kreuzer KN. Coordination of DNA ends during double-strand-break repair in bacteriophage T4. Genetics. 2002 Nov;162(3):1019–30.
Stohr, Bradley A., and Kenneth N. Kreuzer. “Coordination of DNA ends during double-strand-break repair in bacteriophage T4.Genetics, vol. 162, no. 3, Nov. 2002, pp. 1019–30. Pubmed, doi:10.1093/genetics/162.3.1019.
Stohr BA, Kreuzer KN. Coordination of DNA ends during double-strand-break repair in bacteriophage T4. Genetics. 2002 Nov;162(3):1019–1030.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

November 2002

Volume

162

Issue

3

Start / End Page

1019 / 1030

Location

United States

Related Subject Headings

  • Developmental Biology
  • DNA Repair
  • DNA
  • Chromosomes
  • Biological Assay
  • Bacteriophage T4
  • 3105 Genetics
  • 3101 Biochemistry and cell biology
  • 0604 Genetics