Skip to main content

Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase.

Publication ,  Journal Article
Kreuzer, KN
Published in: J Biol Chem
April 25, 1984

The bacteriophage T4-induced type II DNA topoisomerase has been shown previously to make a reversible double strand break in DNA double helices. In addition, this enzyme is shown here to bind tightly and to cleave single-stranded DNA molecules. The evidence that the single-stranded DNA cleavage activity is intrinsic to the topoisomerase includes: 1) protein linkage to the 5' ends of the newly cleaved DNA; 2) coelution of essentially homogeneous topoisomerase and the DNA cleavage activity; 3) inhibition of both single-stranded DNA cleavage and double-stranded DNA relaxation by oxolinic acid; and 4) inhibition of duplex DNA relaxation by single-stranded DNA. The major cleavage sites on phi X174 viral DNA substrates have been mapped, and several cleavage sites analyzed to determine the exact nucleotide position of cleavage. Major cleavage sites are found very near the base of predicted hairpin helices in the single-stranded DNA substrates, suggesting that DNA secondary structure recognition is important in the cleavage reaction. On the other hand, there are also many weaker cleavage sites with no obvious sequence requirements. Many of the properties of the single-stranded DNA cleavage reaction examined here differ from those of the oxolinic acid-dependent, double-stranded DNA cleavage reaction catalyzed by the same enzyme.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1984

Volume

259

Issue

8

Start / End Page

5347 / 5354

Location

United States

Related Subject Headings

  • T-Phages
  • Substrate Specificity
  • Protein Binding
  • Nucleic Acid Conformation
  • Molecular Weight
  • Escherichia coli
  • Enzyme Induction
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type II
  • DNA Restriction Enzymes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kreuzer, K. N. “Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase.J Biol Chem 259, no. 8 (April 25, 1984): 5347–54.
Kreuzer, K. N. “Recognition of single-stranded DNA by the bacteriophage T4-induced type II topoisomerase.J Biol Chem, vol. 259, no. 8, Apr. 1984, pp. 5347–54.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

April 25, 1984

Volume

259

Issue

8

Start / End Page

5347 / 5354

Location

United States

Related Subject Headings

  • T-Phages
  • Substrate Specificity
  • Protein Binding
  • Nucleic Acid Conformation
  • Molecular Weight
  • Escherichia coli
  • Enzyme Induction
  • DNA, Single-Stranded
  • DNA Topoisomerases, Type II
  • DNA Restriction Enzymes