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Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations.

Publication ,  Journal Article
Kreuzer, KN; McEntee, K; Geballe, AP; Cozzarelli, NR
Published in: Mol Gen Genet
November 29, 1978

Defective lambda transducing phages for the nalA region of the Escherichia coli chromosome were isolated from a lysogen in which lambda is inserted in the nearby glpT gene. The three classes of transducing phages designated lambdanrdA, lambdaubiG, and lambdadnalA contained bacterial DNA extending from glpT through nrdA, ubiG, and nalA, respectively. The bacterial genes are in the left arm of the lambda chromosome. Of the eleven polypeptides coded by lambdadnalA that were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate only one was not also specified by lambdadubiG. This 105,000 dalton polypeptide is the nalA gene product. The electorphoretic mobility and isoelectric point of this protein were unaffected by a nalA mutation (nalA48) that confers nalidixic acid resistance. Temperature-sensitive and amber mutations in the nalA gene were isolated using a lambdadnalA48 lysogen which is heterodiploid for nalA. The conditional lethality of these mutations proves that nalA is an essential locus.

Duke Scholars

Published In

Mol Gen Genet

DOI

ISSN

0026-8925

Publication Date

November 29, 1978

Volume

167

Issue

2

Start / End Page

129 / 137

Location

Germany

Related Subject Headings

  • Transduction, Genetic
  • Plant Biology & Botany
  • Nalidixic Acid
  • Mutation
  • Genetics & Heredity
  • Genes, Lethal
  • Escherichia coli
  • Drug Resistance, Microbial
  • Defective Viruses
  • Coliphages
 

Citation

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Kreuzer, K. N., McEntee, K., Geballe, A. P., & Cozzarelli, N. R. (1978). Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol Gen Genet, 167(2), 129–137. https://doi.org/10.1007/BF00266906
Kreuzer, K. N., K. McEntee, A. P. Geballe, and N. R. Cozzarelli. “Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations.Mol Gen Genet 167, no. 2 (November 29, 1978): 129–37. https://doi.org/10.1007/BF00266906.
Kreuzer KN, McEntee K, Geballe AP, Cozzarelli NR. Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol Gen Genet. 1978 Nov 29;167(2):129–37.
Kreuzer, K. N., et al. “Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations.Mol Gen Genet, vol. 167, no. 2, Nov. 1978, pp. 129–37. Pubmed, doi:10.1007/BF00266906.
Kreuzer KN, McEntee K, Geballe AP, Cozzarelli NR. Lambda transducing phages for the nalA gene of Escherichia coli and conditional lethal nalA mutations. Mol Gen Genet. 1978 Nov 29;167(2):129–137.

Published In

Mol Gen Genet

DOI

ISSN

0026-8925

Publication Date

November 29, 1978

Volume

167

Issue

2

Start / End Page

129 / 137

Location

Germany

Related Subject Headings

  • Transduction, Genetic
  • Plant Biology & Botany
  • Nalidixic Acid
  • Mutation
  • Genetics & Heredity
  • Genes, Lethal
  • Escherichia coli
  • Drug Resistance, Microbial
  • Defective Viruses
  • Coliphages