Skip to main content
construction release_alert
Scholars@Duke will be undergoing maintenance April 11-15. Some features may be unavailable during this time.
cancel
Journal cover image

Solution structure of the core NFATC1/DNA complex.

Publication ,  Journal Article
Zhou, P; Sun, LJ; Dötsch, V; Wagner, G; Verdine, GL
Published in: Cell
March 6, 1998

The nuclear factor of the activated T cell (NFAT) family of transcription factors regulates cytokine gene expression by binding to the promoter/enhancer regions of antigen-responsive genes, usually in cooperation with heterologous DNA-binding partners. Here we report the solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the ARRE2 DNA site from the interleukin-2 promoter. The structure reveals that DNA binding induces the folding of key structural elements that are required for both sequence-specific recognition and the establishment of cooperative protein-protein contacts. The orientation of the NFAT DNA-binding domain observed in the binary NFATC1-DBD*/ DNA complex is distinct from that seen in the ternary NFATC2/AP-1/DNA complex, suggesting that the domain reorients upon formation of a cooperative transcriptional complex.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 6, 1998

Volume

92

Issue

5

Start / End Page

687 / 696

Location

United States

Related Subject Headings

  • Transcription Factors
  • Sequence Alignment
  • Protein Conformation
  • Point Mutation
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Nuclear Proteins
  • Nuclear Magnetic Resonance, Biomolecular
  • NFATC Transcription Factors
  • Molecular Sequence Data
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Zhou, P., Sun, L. J., Dötsch, V., Wagner, G., & Verdine, G. L. (1998). Solution structure of the core NFATC1/DNA complex. Cell, 92(5), 687–696. https://doi.org/10.1016/s0092-8674(00)81136-8
Zhou, P., L. J. Sun, V. Dötsch, G. Wagner, and G. L. Verdine. “Solution structure of the core NFATC1/DNA complex.Cell 92, no. 5 (March 6, 1998): 687–96. https://doi.org/10.1016/s0092-8674(00)81136-8.
Zhou P, Sun LJ, Dötsch V, Wagner G, Verdine GL. Solution structure of the core NFATC1/DNA complex. Cell. 1998 Mar 6;92(5):687–96.
Zhou, P., et al. “Solution structure of the core NFATC1/DNA complex.Cell, vol. 92, no. 5, Mar. 1998, pp. 687–96. Pubmed, doi:10.1016/s0092-8674(00)81136-8.
Zhou P, Sun LJ, Dötsch V, Wagner G, Verdine GL. Solution structure of the core NFATC1/DNA complex. Cell. 1998 Mar 6;92(5):687–696.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

March 6, 1998

Volume

92

Issue

5

Start / End Page

687 / 696

Location

United States

Related Subject Headings

  • Transcription Factors
  • Sequence Alignment
  • Protein Conformation
  • Point Mutation
  • Oligodeoxyribonucleotides
  • Nucleic Acid Conformation
  • Nuclear Proteins
  • Nuclear Magnetic Resonance, Biomolecular
  • NFATC Transcription Factors
  • Molecular Sequence Data