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Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele.

Publication ,  Journal Article
Ferrari, G; Neal, W; Ottinger, J; Jones, AM; Edwards, BH; Goepfert, P; Betts, MR; Koup, RA; Buchbinder, S; McElrath, MJ; Tartaglia, J; Weinhold, KJ
Published in: J Immunol
August 1, 2004

According to a number of previous reports, control of HIV replication in humans appears to be linked to the presence of anti-HIV-1 Gag-specific CD8 responses. During the chronic phase of HIV-1 infection, up to 75% of the HIV-infected individuals who express the histocompatibility leukocyte Ag (HLA)-A*0201 recognize the Gag p17 SLYNTVATL (aa residues 77-85) epitope (SL9). However, the role of the anti-SL9 CD8 CTL in controlling HIV-1 infection remains controversial. In this study we determined whether the pattern of SL9 immunodominance in uninfected, HLA-A*0201 HIV vaccine recipients is similar to that seen in chronically HIV-infected subjects. The presence of anti-SL9 responses was determined using a panel of highly sensitive cellular immunoassays, including peptide:MHC tetramer binding, IFN-gamma ELISPOT, and cytokine flow cytometry. Thirteen HLA-A*0201 vaccinees with documented anti-Gag CD8 CTL reactivities were tested, and none had a detectable anti-SL9 response. These findings strongly suggest that the pattern of SL9 epitope immunodominance previously reported among chronically infected, HLA-A*0201-positive patients is not recapitulated in noninfected recipients of Gag-containing canarypox-based candidate vaccines and may be influenced by the relative immunogenicity of these constructs.

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Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

August 1, 2004

Volume

173

Issue

3

Start / End Page

2126 / 2133

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Vaccines, Synthetic
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocytes, Cytotoxic
  • T-Cell Antigen Receptor Specificity
  • Peptide Fragments
  • Immunology
  • Immunodominant Epitopes
  • Immunization, Secondary
 

Citation

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Ferrari, G., Neal, W., Ottinger, J., Jones, A. M., Edwards, B. H., Goepfert, P., … Weinhold, K. J. (2004). Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele. J Immunol, 173(3), 2126–2133. https://doi.org/10.4049/jimmunol.173.3.2126
Ferrari, Guido, Wesley Neal, Janet Ottinger, Anizsa M. Jones, Bradley H. Edwards, Paul Goepfert, Michael R. Betts, et al. “Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele.J Immunol 173, no. 3 (August 1, 2004): 2126–33. https://doi.org/10.4049/jimmunol.173.3.2126.
Ferrari G, Neal W, Ottinger J, Jones AM, Edwards BH, Goepfert P, et al. Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele. J Immunol. 2004 Aug 1;173(3):2126–33.
Ferrari, Guido, et al. “Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele.J Immunol, vol. 173, no. 3, Aug. 2004, pp. 2126–33. Pubmed, doi:10.4049/jimmunol.173.3.2126.
Ferrari G, Neal W, Ottinger J, Jones AM, Edwards BH, Goepfert P, Betts MR, Koup RA, Buchbinder S, McElrath MJ, Tartaglia J, Weinhold KJ. Absence of immunodominant anti-Gag p17 (SL9) responses among Gag CTL-positive, HIV-uninfected vaccine recipients expressing the HLA-A*0201 allele. J Immunol. 2004 Aug 1;173(3):2126–2133.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

August 1, 2004

Volume

173

Issue

3

Start / End Page

2126 / 2133

Location

United States

Related Subject Headings

  • env Gene Products, Human Immunodeficiency Virus
  • Vaccines, Synthetic
  • Vaccines, DNA
  • Vaccination
  • T-Lymphocytes, Cytotoxic
  • T-Cell Antigen Receptor Specificity
  • Peptide Fragments
  • Immunology
  • Immunodominant Epitopes
  • Immunization, Secondary