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Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages.

Publication ,  Journal Article
Bolaños, B; Mitchell, TG
Published in: J Med Vet Mycol
1989

A radiolabeling method was developed to investigate the interaction in vitro between Cryptococcus neoformans and Lewis rat alveolar macrophages (AM phi). AM phi were harvested by lung lavage, monolayers of adherent cells were established in wells of microtiter plates and [51Cr]-labeled yeast cells were added to the monolayers. After removal of extracellular yeasts, the adherent radioactivity associated with the AM phi was directly proportional both to the number of yeasts added and to the number of yeasts per AM phi as determined by microscopic examination of Giemsa-stained monolayers. Phagocytosis (attachment and/or ingestion) of radiolabeled C. neoformans by AM phi was a sensitive, quantitative and reproducible assay for the evaluation of the AM phi-C. neoformans interaction. AM phi were able to phagocytose encapsulated strains of C. neoformans. The extent of phagocytosis was inversely related to the capsule size. Normal rat serum (NRS) was an excellent source of opsonins for the ingestion. Inactivation of serum complement or depletion of C3 by affinity chromatography removed most of the opsonic activity of NRS. Specific antibodies against C. neoformans did not increase phagocytosis.

Duke Scholars

Published In

J Med Vet Mycol

ISSN

0268-1218

Publication Date

1989

Volume

27

Issue

4

Start / End Page

203 / 217

Location

England

Related Subject Headings

  • Rats, Inbred Strains
  • Rats, Inbred Lew
  • Rats
  • Phagocytosis
  • Opsonin Proteins
  • Mycology & Parasitology
  • Male
  • Macrophages
  • Lung
  • Immunoelectrophoresis, Two-Dimensional
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bolaños, B., & Mitchell, T. G. (1989). Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages. J Med Vet Mycol, 27(4), 203–217.
Bolaños, B., and T. G. Mitchell. “Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages.J Med Vet Mycol 27, no. 4 (1989): 203–17.
Bolaños B, Mitchell TG. Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages. J Med Vet Mycol. 1989;27(4):203–17.
Bolaños, B., and T. G. Mitchell. “Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages.J Med Vet Mycol, vol. 27, no. 4, 1989, pp. 203–17.
Bolaños B, Mitchell TG. Phagocytosis of Cryptococcus neoformans by rat alveolar macrophages. J Med Vet Mycol. 1989;27(4):203–217.

Published In

J Med Vet Mycol

ISSN

0268-1218

Publication Date

1989

Volume

27

Issue

4

Start / End Page

203 / 217

Location

England

Related Subject Headings

  • Rats, Inbred Strains
  • Rats, Inbred Lew
  • Rats
  • Phagocytosis
  • Opsonin Proteins
  • Mycology & Parasitology
  • Male
  • Macrophages
  • Lung
  • Immunoelectrophoresis, Two-Dimensional