A compensatory base change in human U2 snRNA can suppress a branch site mutation.
Publication
, Journal Article
Zhuang, Y; Weiner, AM
Published in: Genes Dev
October 1989
We have developed an assay to test whether U2 snRNA can base-pair with the branch site during mammalian mRNA splicing. The beta 110 point mutation (GG----AG) within the first intron of human beta-globin generates a new 3' splice site that is preferentially used. We show here that use of the normal 3' splice site can be restored either by improving the match of a cryptic branch site to the branch site consensus or by introducing mutant U2 snRNAs with greater complementarity to the cryptic branch site. These data indicate that human U2 snRNA can form base pairs with the mRNA precursor; however, base pairing appears to be optional because some mammalian branch sites do not match the consensus.
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Published In
Genes Dev
DOI
ISSN
0890-9369
Publication Date
October 1989
Volume
3
Issue
10
Start / End Page
1545 / 1552
Location
United States
Related Subject Headings
- Transfection
- Suppression, Genetic
- Sequence Homology, Nucleic Acid
- RNA, Small Nuclear
- RNA Splicing
- RNA Precursors
- Mutation
- Molecular Sequence Data
- Humans
- Hela Cells
Citation
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Chicago
ICMJE
MLA
NLM
Zhuang, Y., & Weiner, A. M. (1989). A compensatory base change in human U2 snRNA can suppress a branch site mutation. Genes Dev, 3(10), 1545–1552. https://doi.org/10.1101/gad.3.10.1545
Zhuang, Y., and A. M. Weiner. “A compensatory base change in human U2 snRNA can suppress a branch site mutation.” Genes Dev 3, no. 10 (October 1989): 1545–52. https://doi.org/10.1101/gad.3.10.1545.
Zhuang Y, Weiner AM. A compensatory base change in human U2 snRNA can suppress a branch site mutation. Genes Dev. 1989 Oct;3(10):1545–52.
Zhuang, Y., and A. M. Weiner. “A compensatory base change in human U2 snRNA can suppress a branch site mutation.” Genes Dev, vol. 3, no. 10, Oct. 1989, pp. 1545–52. Pubmed, doi:10.1101/gad.3.10.1545.
Zhuang Y, Weiner AM. A compensatory base change in human U2 snRNA can suppress a branch site mutation. Genes Dev. 1989 Oct;3(10):1545–1552.
Published In
Genes Dev
DOI
ISSN
0890-9369
Publication Date
October 1989
Volume
3
Issue
10
Start / End Page
1545 / 1552
Location
United States
Related Subject Headings
- Transfection
- Suppression, Genetic
- Sequence Homology, Nucleic Acid
- RNA, Small Nuclear
- RNA Splicing
- RNA Precursors
- Mutation
- Molecular Sequence Data
- Humans
- Hela Cells