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The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.

Publication ,  Journal Article
Zhuang, Y; Leung, H; Weiner, AM
Published in: Mol Cell Biol
August 1987

The use of alternative 5' splice sites in the simian virus 40 early-transcription unit controls the ratio of large T to small t antigen during viral infection. To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence. Whether these mutants were assayed in vitro or in vivo, we found that the efficiency of large-T splicing is increased by improving the match of the large-T-antigen 5' splice site to the consensus. We conclude that the match of a 5' splice site is an important determinant of 5' splice site utilization and that the simian virus 40 large-T-antigen 5' splice site is almost certainly recognized by the U1 small nuclear RNA component of the U1 small nuclear ribonucleoprotein particle.

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

August 1987

Volume

7

Issue

8

Start / End Page

3018 / 3020

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Simian virus 40
  • RNA, Small Nuclear
  • RNA Splicing
  • RNA Precursors
  • Protein Kinases
  • Genes, Viral
  • Genes
  • Developmental Biology
  • Base Sequence
 

Citation

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Zhuang, Y., Leung, H., & Weiner, A. M. (1987). The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA. Mol Cell Biol, 7(8), 3018–3020. https://doi.org/10.1128/mcb.7.8.3018-3020.1987
Zhuang, Y., H. Leung, and A. M. Weiner. “The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.Mol Cell Biol 7, no. 8 (August 1987): 3018–20. https://doi.org/10.1128/mcb.7.8.3018-3020.1987.
Zhuang, Y., et al. “The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.Mol Cell Biol, vol. 7, no. 8, Aug. 1987, pp. 3018–20. Pubmed, doi:10.1128/mcb.7.8.3018-3020.1987.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

August 1987

Volume

7

Issue

8

Start / End Page

3018 / 3020

Location

United States

Related Subject Headings

  • Transcription, Genetic
  • Simian virus 40
  • RNA, Small Nuclear
  • RNA Splicing
  • RNA Precursors
  • Protein Kinases
  • Genes, Viral
  • Genes
  • Developmental Biology
  • Base Sequence