The natural 5' splice site of simian virus 40 large T antigen can be improved by increasing the base complementarity to U1 RNA.
The use of alternative 5' splice sites in the simian virus 40 early-transcription unit controls the ratio of large T to small t antigen during viral infection. To study the regulation of these alternative 5' splice sites, we made two mutants which improve the match of the large-T-antigen 5' splice site to the 5' splice site consensus sequence. Whether these mutants were assayed in vitro or in vivo, we found that the efficiency of large-T splicing is increased by improving the match of the large-T-antigen 5' splice site to the consensus. We conclude that the match of a 5' splice site is an important determinant of 5' splice site utilization and that the simian virus 40 large-T-antigen 5' splice site is almost certainly recognized by the U1 small nuclear RNA component of the U1 small nuclear ribonucleoprotein particle.
Duke Scholars
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- Transcription, Genetic
- Simian virus 40
- RNA, Small Nuclear
- RNA Splicing
- RNA Precursors
- Protein Kinases
- Genes, Viral
- Genes
- Developmental Biology
- Base Sequence
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transcription, Genetic
- Simian virus 40
- RNA, Small Nuclear
- RNA Splicing
- RNA Precursors
- Protein Kinases
- Genes, Viral
- Genes
- Developmental Biology
- Base Sequence