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Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice.

Publication ,  Journal Article
Ding, S; Wu, X; Li, G; Han, M; Zhuang, Y; Xu, T
Published in: Cell
August 12, 2005

Transposable elements have been routinely used for genetic manipulation in lower organisms, including generating transgenic animals and insertional mutagenesis. In contrast, the usage of transposons in mice and other vertebrate systems is still limited due to the lack of an efficient transposition system. We have tested the ability of piggyBac (PB), a DNA transposon from the cabbage looper moth Trichoplusia ni, to transpose in mammalian systems. We show that PB elements carrying multiple genes can efficiently transpose in human and mouse cell lines and also in mice. PB permits the expression of the marker genes it carried. During germline transposition, PB could excise precisely from original insertion sites and transpose into the mouse genome at diverse locations, preferably transcription units. These data provide a first and critical step toward a highly efficient transposon system for a variety of genetic manipulations including transgenesis and insertional mutagenesis in mice and other vertebrates.

Duke Scholars

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Published In

Cell

DOI

ISSN

0092-8674

Publication Date

August 12, 2005

Volume

122

Issue

3

Start / End Page

473 / 483

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Mutagenesis
  • Mice, Transgenic
  • Mice
  • Humans
  • Genetic Vectors
  • Gene Expression Regulation
  • Developmental Biology
  • DNA Transposable Elements
  • Cell Line
 

Citation

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Ding, S., Wu, X., Li, G., Han, M., Zhuang, Y., & Xu, T. (2005). Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell, 122(3), 473–483. https://doi.org/10.1016/j.cell.2005.07.013
Ding, Sheng, Xiaohui Wu, Gang Li, Min Han, Yuan Zhuang, and Tian Xu. “Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice.Cell 122, no. 3 (August 12, 2005): 473–83. https://doi.org/10.1016/j.cell.2005.07.013.
Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell. 2005 Aug 12;122(3):473–83.
Ding, Sheng, et al. “Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice.Cell, vol. 122, no. 3, Aug. 2005, pp. 473–83. Pubmed, doi:10.1016/j.cell.2005.07.013.
Ding S, Wu X, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell. 2005 Aug 12;122(3):473–483.
Journal cover image

Published In

Cell

DOI

ISSN

0092-8674

Publication Date

August 12, 2005

Volume

122

Issue

3

Start / End Page

473 / 483

Location

United States

Related Subject Headings

  • Transformation, Genetic
  • Mutagenesis
  • Mice, Transgenic
  • Mice
  • Humans
  • Genetic Vectors
  • Gene Expression Regulation
  • Developmental Biology
  • DNA Transposable Elements
  • Cell Line