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Genetic basis for p53 overexpression in human breast cancer.

Publication ,  Journal Article
Davidoff, AM; Humphrey, PA; Iglehart, JD; Marks, JR
Published in: Proc Natl Acad Sci U S A
June 1, 1991

Overexpression of an activated form of the p53 protein may be involved in neoplastic transformation. We found widespread overexpression of p53 by immunohistochemical staining in 11 (22%) of 49 primary invasive human breast cancers. Northern blot analysis showed that this overexpression was not due to an increase in the steady-state level of p53 mRNA. The p53 gene was directly sequenced in 7 of these tumors with elevated levels of the protein and, in each case, a mutation that altered the coding sequence for p53 was found in a highly conserved region of the gene. Whereas 4 of these tumors contained only a mutant p53 allele, the other 3 tumors exhibited coding sequences from both a mutant and a wild-type allele. p53 mutations have previously been correlated with allelic loss of part of chromosome 17p that contains the p53 locus. Examination of all 49 breast tumors revealed a 61% frequency of deletion at or near the p53 locus. However, the presence of allelic deletion did not correlate with overexpression of the protein. Six tumors that were deleted but did not express high levels of the protein were sequenced and all retained a wild-type p53 allele. In this series of human breast cancers, overexpression of the p53 protein, not allelic loss on chromosome 17p, was always associated with mutation of the p53 gene.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1, 1991

Volume

88

Issue

11

Start / End Page

5006 / 5010

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Restriction Mapping
  • RNA, Neoplasm
  • RNA, Messenger
  • Oligonucleotide Probes
  • Mutation
  • Molecular Sequence Data
  • Immunohistochemistry
  • Humans
  • Gene Expression
 

Citation

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Davidoff, A. M., Humphrey, P. A., Iglehart, J. D., & Marks, J. R. (1991). Genetic basis for p53 overexpression in human breast cancer. Proc Natl Acad Sci U S A, 88(11), 5006–5010. https://doi.org/10.1073/pnas.88.11.5006
Davidoff, A. M., P. A. Humphrey, J. D. Iglehart, and J. R. Marks. “Genetic basis for p53 overexpression in human breast cancer.Proc Natl Acad Sci U S A 88, no. 11 (June 1, 1991): 5006–10. https://doi.org/10.1073/pnas.88.11.5006.
Davidoff AM, Humphrey PA, Iglehart JD, Marks JR. Genetic basis for p53 overexpression in human breast cancer. Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):5006–10.
Davidoff, A. M., et al. “Genetic basis for p53 overexpression in human breast cancer.Proc Natl Acad Sci U S A, vol. 88, no. 11, June 1991, pp. 5006–10. Pubmed, doi:10.1073/pnas.88.11.5006.
Davidoff AM, Humphrey PA, Iglehart JD, Marks JR. Genetic basis for p53 overexpression in human breast cancer. Proc Natl Acad Sci U S A. 1991 Jun 1;88(11):5006–5010.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

June 1, 1991

Volume

88

Issue

11

Start / End Page

5006 / 5010

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Restriction Mapping
  • RNA, Neoplasm
  • RNA, Messenger
  • Oligonucleotide Probes
  • Mutation
  • Molecular Sequence Data
  • Immunohistochemistry
  • Humans
  • Gene Expression