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Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain).

Publication ,  Journal Article
Mercer, JA; Marks, JR; Spector, DH
Published in: Virology
August 1983

We have cloned EcoRI and HindIII fragments of the Smith strain of murine cytomegalovirus (MCMV) in the plasmid vector pACYC184. These cloned fragments were used to establish a restriction endonuclease map of the genome with respect to the EcoRI and HindIII sites. The map was constructed on the basis of data derived from cross-hybridizations of EcoRI and HindIII cloned fragments, double-digestions of the cloned fragments with EcoRI and HindIII, and hybridization of cloned HindIII fragments to Southern blots of MCMV DNA cleaved with EcoRI. From our mapping data, we have determined that the length of the MCMV genome is approximately 240 kbp. The genome does not appear to undergo inversions and lacks detectable repeated sequences. One HindIII cloned fragment was obtained which contained both HindIII termini. The existence of this fragment may be related to the mode of replication of the MCMV genome.

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Published In

Virology

DOI

ISSN

0042-6822

Publication Date

August 1983

Volume

129

Issue

1

Start / End Page

94 / 106

Location

United States

Related Subject Headings

  • Virology
  • Nucleic Acid Hybridization
  • Mice
  • Genes, Viral
  • Deoxyribonuclease HindIII
  • Deoxyribonuclease EcoRI
  • DNA, Viral
  • DNA Restriction Enzymes
  • Cytomegalovirus
  • Cloning, Molecular
 

Citation

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Mercer, J. A., Marks, J. R., & Spector, D. H. (1983). Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain). Virology, 129(1), 94–106. https://doi.org/10.1016/0042-6822(83)90398-7
Mercer, J. A., J. R. Marks, and D. H. Spector. “Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain).Virology 129, no. 1 (August 1983): 94–106. https://doi.org/10.1016/0042-6822(83)90398-7.
Mercer, J. A., et al. “Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain).Virology, vol. 129, no. 1, Aug. 1983, pp. 94–106. Pubmed, doi:10.1016/0042-6822(83)90398-7.

Published In

Virology

DOI

ISSN

0042-6822

Publication Date

August 1983

Volume

129

Issue

1

Start / End Page

94 / 106

Location

United States

Related Subject Headings

  • Virology
  • Nucleic Acid Hybridization
  • Mice
  • Genes, Viral
  • Deoxyribonuclease HindIII
  • Deoxyribonuclease EcoRI
  • DNA, Viral
  • DNA Restriction Enzymes
  • Cytomegalovirus
  • Cloning, Molecular